Back to Search
Start Over
Effects of cigarette smoke and nicotine on cell viability, migration and myofibroblastic differentiation.
- Source :
-
Journal of periodontal research [J Periodontal Res] 2012 Oct; Vol. 47 (5), pp. 599-607. - Publication Year :
- 2012
-
Abstract
- Background and Objective: Several studies have analysed the role of nicotine as a prominent agent affecting wound repair in smokers. However, tobacco smoke contains several components that may alter gingival wound healing. The present study aimed to analyse the roles of cigarette smoke condensate (CSC) and nicotine on cell viability, cell migration/invasion and myofibroblastic differentiation using primary cultures of human gingival fibroblasts.<br />Material and Methods: To compare the effects of CSC and nicotine, gingival fibroblasts were stimulated with CSC (0.4–500 lg/mL) and the corresponding nicotine concentrations (0.025–32 lg/mL) present in research cigarettes (1R3F). Cell viability was evaluated through the MTS assay. Cell migration and invasion were assessed through scratch wound assays, collagen nested matrices and trans well migration. a-Smooth muscle actin production was evaluated by western blotting.<br />Results: Cigarette smoke condensate at 50 lg/mL induced a moderate increase in cell viability, whereas the corresponding nicotine concentration (3.2 lg/mL) did not produce this response. Cigarette smoke condensate at 250 lg/mL, but not nicotine at 16 lg/mL (the corresponding nicotine concentration), induced cell death. Both nicotine and CSC stimulated cell migration (50 lg/mL CSC; 3.2 lg/mL nicotine). At 150 lg/mL, CSC inhibited cell migration; however, the corresponding concentration of nicotine (9.6 lg/mL), did not have this effect. Although both nicotine and CSC inhibited a-smooth muscle actin production, only the latter induced a statistically significant effect on this response.<br />Conclusion: Cigarette smoke condensate may stimulate cell survival and migration at low concentrations and inhibit these cell responses at higher levels of exposure. Moreover, CSC may interfere in myofibroblastic differentiation.These results show that cigarette smoke, but not nicotine, may significantly alter cell viability, cell migration and myofibroblastic differentiation in gingival mesenchymal cells.
- Subjects :
- Actins drug effects
Cell Culture Techniques
Cell Death drug effects
Cell Differentiation drug effects
Cell Movement drug effects
Cell Survival drug effects
Cells, Cultured
Coloring Agents
Fibroblasts drug effects
Gingiva cytology
Humans
Tetrazolium Salts
Thiazoles
Gingiva drug effects
Myofibroblasts drug effects
Nicotine pharmacology
Nicotinic Agonists pharmacology
Smoke adverse effects
Nicotiana adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0765
- Volume :
- 47
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of periodontal research
- Publication Type :
- Academic Journal
- Accession number :
- 23091836
- Full Text :
- https://doi.org/10.1111/j.1600-0765.2012.01472.x