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Bioassays against pinewood nematode: assessment of a suitable dilution agent and screening for bioactive essential oils.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2012 Oct 19; Vol. 17 (10), pp. 12312-29. Date of Electronic Publication: 2012 Oct 19. - Publication Year :
- 2012
-
Abstract
- Acetone was investigated and found to be an appropriate alternative to Triton X-100 as a solvent of essential oils in bioassays aimed to investigate their effects on pinewood nematode (Bursaphelenchus xylophilus) mortality. Therefore it was used as dilution agent to screen the effectiveness of fifty two essential oils against this pest. Thirteen essential oils were highly effective, resulting in more than 90% pinewood nematode mortality at 2 mg/mL, with six of them resulting in 100% mortality. LC₁₀₀ values ranged between 0.50 mg/mL and 0.83 mg/mL for the essential oils of Origanum vulgare and Satureja montana, respectively. Essential oils were submitted to gas chromatography and gas chromatography-mass spectrometry analysis and their chemical composition established. Data from essential oils with 100% mortality at 2 mg/mL and other essential oils previously found to have LC₁₀₀ ≤ 2 mg/mL was combined, their chemical profiles investigated by correspondences analysis plus automatic classification.
- Subjects :
- Acetone chemistry
Animals
Antinematodal Agents chemistry
Octoxynol chemistry
Oils, Volatile chemistry
Origanum chemistry
Parasitic Sensitivity Tests methods
Plant Oils chemistry
Plant Oils pharmacology
Satureja chemistry
Solvents chemistry
Antinematodal Agents pharmacology
Nematoda drug effects
Oils, Volatile pharmacology
Toxicity Tests methods
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 17
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 23085666
- Full Text :
- https://doi.org/10.3390/molecules171012312