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Detection and genetic characterization of norovirus in environmental water samples in Thailand.
- Source :
-
The Southeast Asian journal of tropical medicine and public health [Southeast Asian J Trop Med Public Health] 2012 Mar; Vol. 43 (2), pp. 323-32. - Publication Year :
- 2012
-
Abstract
- The aim of this study was to detect and characterize noroviruses (NoVs) in environmental water samples. One hundred and fourteen water samples were collected from a river and irrigation canals in central Thailand during 2006-2007. NoVs were detected by RT-nested PCR in 13% of the samples. The river samples (22%) contained NoVs at a higher frequency than the irrigation canal samples (4%). Among the 15 NoV-positive samples, 9 harbored genogroup (G) I, 2 samples with GII, and 4 samples with mixed GI and GII. DNA sequencing of PCR amplicons and phylogenetic analysis of partial capsid gene revealed that 5 samples were of genotype GI-2, 1 sample was GI-6, and 1 sample was a mix of GI-2 and GII-unclassified genotypes. NoVs in water samples quantified using quantitative RT-PCR were in the range of 4.91 x 10(2) -1.26 x 10(3) copies/ml for NoV GI and 3.51 x 10(3) copies/ml for NoV GII. This is the first study demonstrating the presence of NoV variants in water samples collected from a river and the adjacent canals of Thailand.
Details
- Language :
- English
- ISSN :
- 0125-1562
- Volume :
- 43
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Southeast Asian journal of tropical medicine and public health
- Publication Type :
- Academic Journal
- Accession number :
- 23082584