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Ratiometric activatable cell-penetrating peptides provide rapid in vivo readout of thrombin activation.

Authors :
Whitney M
Savariar EN
Friedman B
Levin RA
Crisp JL
Glasgow HL
Lefkowitz R
Adams SR
Steinbach P
Nashi N
Nguyen QT
Tsien RY
Source :
Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2013 Jan 02; Vol. 52 (1), pp. 325-30. Date of Electronic Publication: 2012 Oct 18.
Publication Year :
2013

Abstract

In real time: thrombin activation in vivo can be imaged in real time with ratiometric activatable cell penetrating peptides (RACPPs). RACPPs are designed to combine 1) dual-emission ratioing, 2) far red to infrared wavelengths for in vivo mammalian imaging, and 3) cleavage-dependent spatial localization. The most advanced RACPP uses norleucine (Nle)-TPRSFL as a linker that increases sensitivity to thrombin by about 90-fold.<br /> (Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Details

Language :
English
ISSN :
1521-3773
Volume :
52
Issue :
1
Database :
MEDLINE
Journal :
Angewandte Chemie (International ed. in English)
Publication Type :
Academic Journal
Accession number :
23080482
Full Text :
https://doi.org/10.1002/anie.201205721