Identification of dairy lactic acid bacteria by tRNAAla-23S rDNA-RFLP.

Authors :: Mancini A
Lazzi C
Bernini V
Neviani E
Gatti M
Source :: Journal of microbiological methods [J Microbiol Methods] 2012 Dec; Vol. 91 (3), pp. 380-90. Date of Electronic Publication: 2012 Oct 16.
Publication Year :: 2012
Abstract: The aim of this study was to evaluate the potential of target tRNA(Ala)-23S ribosomal DNA for identification of lactic acid bacteria strains associated with dairy ecosystem. For this purpose tRNA(Ala)-23S ribosomal DNA Restriction Fragment Length Polymorphism (tRNA(Ala)-23S rDNA-RFLP) was compared with two widely used DNA fingerprinting methods - P1 Random Amplified Polymorphic DNA (RAPD), (GTG)5 repetitive extragenic palindromic PCR (rep-PCR) - for their ability to identify different species on a set of 10 type and 34 reference strains. Moreover, 75 unknown isolates collected during different stages of Grana Padano cheese production and ripening were identified using tRNA(Ala)-23S rDNA-RFLP and compared to the RFLP profiles of the strains in the reference database. This study demonstrated that the target tRNA(Ala)-23S rDNA has high potential in bacterial identification and tRNA(Ala)-23S rDNA-RFLP is a promising method for reliable species-level identification of lactic acid bacteria (LAB) in dairy products.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)