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Human rTNF alpha augments anti-bacterial resistance in mice: potentiation of its effects by recombinant human rIL-1 alpha.
- Source :
-
Immunology [Immunology] 1990 Feb; Vol. 69 (2), pp. 316-22. - Publication Year :
- 1990
-
Abstract
- Treatment with human recombinant tumour necrosis factor-alpha (rTNF alpha) significantly enhanced resistance to Listeria monocytogenes infection in mice. The level of protection (which was dose-dependent and maximal at approximately 1.0 microgram per mouse) was similar to that previously reported for the monokine rIL-1 alpha, although somewhat greater amounts of rTNF alpha than rIL-1 alpha were required. Combined administration of suboptimal concentrations of rTNF alpha and rIL-1 alpha resulted in significant enhancement of resistance beyond that obtained with either monokine alone, whereas further increases in anti-listeria resistance were not observed at doses of rTNF alpha or IL-1 alpha that were themselves capable of inducing substantial protection. Combined administration of rTNF alpha and rIL-1 alpha was associated with a delay in onset and lessening in severity of the lymphopenia that accompanied L. monocytogenes infection. The reduced bacterial burden in the spleens and livers of mice treated with rTNF alpha and rIL-1 alpha was associated with a more rapid decline in serum colony-stimulating activity. Peritoneal macrophages from rTNF alpha- and rIL-1 alpha-treated listeria-infected mice did not demonstrate enhanced anti-listeria activity in vitro. These results provide further evidence for the potential benefits of rTNF alpha and other cytokines in promoting anti-bacterial resistance. They further suggest that use of combinations of cytokines is a strategy worthy of further consideration.
- Subjects :
- Animals
Colony-Stimulating Factors blood
Drug Synergism
Immunity, Innate drug effects
Leukocyte Count drug effects
Listeriosis blood
Listeriosis immunology
Macrophages drug effects
Male
Mice
Recombinant Proteins therapeutic use
Interleukin-1 therapeutic use
Listeriosis prevention & control
Tumor Necrosis Factor-alpha therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 0019-2805
- Volume :
- 69
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 2307486