Real-time PCRs assay for serogrouping Listeria monocytogenes and differentiation from other Listeria spp.

Authors :: Vitullo M
Grant KA
Sammarco ML
Tamburro M
Ripabelli G
Amar CF
Source :: Molecular and cellular probes [Mol Cell Probes] 2013 Feb; Vol. 27 (1), pp. 68-70. Date of Electronic Publication: 2012 Oct 09.
Publication Year :: 2013
Abstract: A 5'-exonuclease real-time triplex-PCR assay was developed for serogrouping Listeria monocytogenes, and differentiation from other Listeria spp. The assay was evaluated on 109 Listeria cultures, and results were compared with a previously validated gel-based multiplex-PCR procedure. All L. monocytogenes were correctly classified into four serogroups, including atypical serotype 4b strains, and differentiated from other Listeria species. The assay is a rapid method for categorisation of suspect L. monocytogenes.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)

Subjects :: Bacterial Proteins genetics
DNA, Bacterial analysis
Food Microbiology
Listeria monocytogenes isolation & purification
Serotyping methods
Species Specificity
Bacterial Typing Techniques methods
Listeria monocytogenes classification
Listeria monocytogenes genetics
Real-Time Polymerase Chain Reaction methods

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