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All-trans retinoic acid combined with 5-Aza-2'-deoxycitidine induces C/EBPα expression and growth inhibition in MLL-AF9-positive leukemic cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2012 Nov 16; Vol. 428 (2), pp. 216-23. Date of Electronic Publication: 2012 Oct 10. - Publication Year :
- 2012
-
Abstract
- The present study tested whether all-trans retinoic acid (ATRA) and 5-Aza-2'-deoxycitidine (5-Aza) affect AML cell differentiation and growth in vitro by acting on the CCAAT/enhancer binding protein α (C/EBPα) and c-Myc axis. After exposure to a combination of these agents, cell differentiation and growth arrest were significantly higher in human and murine MLL-AF9-expressing cells than in MLL-AF4/AF5q31-expressing cells, which were partly associated with increased expression of C/EBPα, C/EBPε, and PU.1, and decreased expression of c-Myc. These findings indicate that MLL-AF9-expressing cells are more sensitive to ATRA and 5-Aza, indicating that different MLL fusion proteins possess different epigenetic properties associated with retinoic acid pathway inactivation.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Line, Tumor
DNA Methylation
Hematopoietic Stem Cells drug effects
Histone-Lysine N-Methyltransferase
Humans
Leukemia, Myeloid, Acute genetics
Mice
Myeloid-Lymphoid Leukemia Protein analysis
Myeloid-Lymphoid Leukemia Protein genetics
Nuclear Proteins analysis
Nuclear Proteins genetics
Oncogene Proteins, Fusion analysis
Oncogene Proteins, Fusion genetics
Azacitidine pharmacology
CCAAT-Enhancer-Binding Protein-alpha biosynthesis
Cell Proliferation drug effects
Drug Resistance, Neoplasm
Leukemia, Myeloid, Acute metabolism
Tretinoin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 428
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 23063977
- Full Text :
- https://doi.org/10.1016/j.bbrc.2012.09.131