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All-trans retinoic acid combined with 5-Aza-2'-deoxycitidine induces C/EBPα expression and growth inhibition in MLL-AF9-positive leukemic cells.

Authors :
Fujiki A
Imamura T
Sakamoto K
Kawashima S
Yoshida H
Hirashima Y
Miyachi M
Yagyu S
Nakatani T
Sugita K
Hosoi H
Source :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2012 Nov 16; Vol. 428 (2), pp. 216-23. Date of Electronic Publication: 2012 Oct 10.
Publication Year :
2012

Abstract

The present study tested whether all-trans retinoic acid (ATRA) and 5-Aza-2'-deoxycitidine (5-Aza) affect AML cell differentiation and growth in vitro by acting on the CCAAT/enhancer binding protein α (C/EBPα) and c-Myc axis. After exposure to a combination of these agents, cell differentiation and growth arrest were significantly higher in human and murine MLL-AF9-expressing cells than in MLL-AF4/AF5q31-expressing cells, which were partly associated with increased expression of C/EBPα, C/EBPε, and PU.1, and decreased expression of c-Myc. These findings indicate that MLL-AF9-expressing cells are more sensitive to ATRA and 5-Aza, indicating that different MLL fusion proteins possess different epigenetic properties associated with retinoic acid pathway inactivation.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1090-2104
Volume :
428
Issue :
2
Database :
MEDLINE
Journal :
Biochemical and biophysical research communications
Publication Type :
Academic Journal
Accession number :
23063977
Full Text :
https://doi.org/10.1016/j.bbrc.2012.09.131