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Expanded HIV-1 cellular tropism by phenotypic mixing with murine endogenous retroviruses.
- Source :
-
Science (New York, N.Y.) [Science] 1990 Feb 16; Vol. 247 (4944), pp. 848-52. - Publication Year :
- 1990
-
Abstract
- In view of the current interest in in vivo murine models for acquired immunodeficiency syndrome (AIDS), the interaction between human immunodeficiency virus type 1 (HIV-1) and endogenous murine leukemia virus (MuLV)-related retroviruses was investigated with a human leukemic T cell line (PF-382x) that acquired xenotropic MuLV (X-MuLV) after in vivo passage in immunosuppressed mice. Despite similar levels of membrane CD4 expression and HIV-1 125I-labeled gp 120 binding, a dramatic acceleration in the time course of HIV-1 infection was observed in PF-382x compared to its X-MuLV-negative counterpart (PF-382). Moreover, PF-382 cells coinfected by X-MuLV and HIV-1 generated a progeny of phenotypically mixed viral particles, enabling HIV-1 to productively infect a panel of CD4- human cells, including B lymphoid cells and purified normal peripheral blood CD4-/CD8+ T lymphocytes. Mixed viral phenotypes were also produced by human CD4+ T cells coinfected with an amphotropic MuLV-related retrovirus (A-MuLV) and HIV-1. These data show that endogenous MuLV acquired by human cells transplanted into mice can significantly interact with HIV-1, thereby inducing important alterations of HIV-1 biological properties.
- Subjects :
- Acquired Immunodeficiency Syndrome immunology
Animals
Antibodies, Monoclonal
CD4 Antigens analysis
Cell Line
Cell Transformation, Viral
Disease Models, Animal
HIV-1 physiology
Hematopoietic Stem Cells cytology
Hematopoietic Stem Cells microbiology
Humans
Mice
Phenotype
Viral Proteins analysis
Virus Replication
HIV-1 genetics
Retroviridae genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0036-8075
- Volume :
- 247
- Issue :
- 4944
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 2305256
- Full Text :
- https://doi.org/10.1126/science.2305256