Measurement of glutathione transferases.

Authors :: Mannervik B
Jemth P
Source :: Current protocols in toxicology [Curr Protoc Toxicol] 2001; Vol. Chapter 6, pp. Unit6.4.
Publication Year :: 2001
Abstract: There are multiple glutathione transferase genes, the proteins for which have different substrate specificities. The various genes are differentially expressed such that species and organs and tissues differ qualitatively and quantitatively for cytosolic and membrane-bound forms. This unit provides protocols for analysis of transferase activity in a continuous spectrophotometric assay and an assay with dichloromethane as the substrate.