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Overexpression of a Paenibacillus campinasensis xylanase in Bacillus megaterium and its applications to biobleaching of cotton stalk pulp and saccharification of recycled paper sludge.
- Source :
-
Bioresource technology [Bioresour Technol] 2012 Dec; Vol. 125, pp. 182-7. Date of Electronic Publication: 2012 Sep 05. - Publication Year :
- 2012
-
Abstract
- A xylanase gene (xynG1-1) from Paenibacillus campinasensis G1-1 was expressed in Bacillus megaterium MS941 and a high level of extracellular xylansae activity (304.26 IU/mL) was achieved after induction with 0.5% xylose. The purified recombinant xylanase (XynG1-1R) revealed optimal activity at 60°C and pH 7.0 and retained 79% and 81% activity after incubation without substrate at 60°C, pH 5.0 and pH 8.0 for 3h, respectively. Application of XynG1-1R (15 IU/g pulp) to cotton stalk pulp bleaching increased brightness by 3.65% over that of the control without the xylanase and reduced the need for chlorine compounds by 50% without loss of brightness and pulp fiber qualities. When XynG1-1R (80 IU/g paper sludge) was used in combination with mixed cellulolytic enzymes, the saccharification efficiency of recycled paper sludge was increased by 10%. These results indicated that XynG1-1R is a promising candidate for various industrial applications such as biobleaching and bioenergy conversion.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Subjects :
- Bacillus megaterium genetics
Color
Endo-1,4-beta Xylanases genetics
Paenibacillus genetics
Plant Components, Aerial microbiology
Plant Extracts metabolism
Protein Engineering methods
Recycling
Up-Regulation
Bacillus megaterium enzymology
Endo-1,4-beta Xylanases metabolism
Gossypium microbiology
Paenibacillus enzymology
Paper
Sewage microbiology
Xylose biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 125
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 23026332
- Full Text :
- https://doi.org/10.1016/j.biortech.2012.08.101