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Impact of protease inhibitors on dentin matrix degradation by collagenase.
- Source :
-
Journal of dental research [J Dent Res] 2012 Dec; Vol. 91 (12), pp. 1119-23. Date of Electronic Publication: 2012 Sep 28. - Publication Year :
- 2012
-
Abstract
- This proof-of-concept study assessed whether the reduction of the degradation of the demineralized organic matrix (DOM) by pre-treatment with protease inhibitors (PI) is effective against dentin matrix loss. Bovine dentin slices were demineralized with 0.87 M citric acid, pH 2.3, for 36 hrs. In sequence, specimens were treated or not (UT, untreated) for 1 min with gels containing epigallocatechin 3-gallate (EGCG, 400 µM), chlorhexidine (CHX, 0.012%), FeSO(4) (1 mM), NaF (1.23%), or no active compound (P, placebo). Specimens were then stored in artificial saliva (5 days, 37°C) with the addition of collagenase (Clostridium histolyticum, 100 U/mL). We analyzed collagen degradation by assaying hydroxyproline (HYP) in the incubation solutions (n = 5) and evaluated the dentin matrix loss by profilometry (n = 12). Data were analyzed by ANOVA and Tukey's test (p < 0.05). Treatment with gels containing EGCG, CHX, or FeSO(4) led to significantly lower HYP concentrations in solution and dentin matrix loss when compared with the other treatments. These results strongly suggest that the preventive effects of the PI tested against dentin erosion are due to their ability to reduce the degradation of the DOM.
- Subjects :
- Analysis of Variance
Animals
Bacterial Proteins metabolism
Catechin analogs & derivatives
Catechin therapeutic use
Cattle
Chlorhexidine therapeutic use
Dentin ultrastructure
Extracellular Matrix metabolism
Ferrous Compounds therapeutic use
Sodium Fluoride therapeutic use
Tooth Demineralization enzymology
Tooth Demineralization prevention & control
Tooth Erosion enzymology
Cariostatic Agents therapeutic use
Collagen metabolism
Collagenases metabolism
Dentin metabolism
Protease Inhibitors therapeutic use
Tooth Erosion prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1544-0591
- Volume :
- 91
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of dental research
- Publication Type :
- Academic Journal
- Accession number :
- 23023765
- Full Text :
- https://doi.org/10.1177/0022034512455801