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Mesenchymal stem cells regulate the proliferation of T cells via the growth-related oncogene/CXC chemokine receptor, CXCR2.
- Source :
-
Cellular immunology [Cell Immunol] 2012 Sep; Vol. 279 (1), pp. 1-11. Date of Electronic Publication: 2012 Sep 05. - Publication Year :
- 2012
-
Abstract
- Mesenchymal stem cells (MSCs) have known to induce immunosuppressive properties by preventing T cell proliferation. However, it is remains unclear how MSCs inhibit T cell proliferation. To identify the factor that inhibits T cell proliferation, we conducted a cytokine array analysis of culture medium from a co-culture of MSCs and T cells and found that the chemokines, CXCL1, 2 and 3, were induced in T cells. MSCs also induced the expression of the CXCR2 receptor on T cell surface. Particularly, CXCL3 inhibited proliferation and increased apoptosis in T cells, which were reversed by CXCR2 inhibitor treatment. Moreover, CXCL3 decreased JAK2, STAT3, and AKT phosphorylation and these responses were also abolished by CXCR2 inhibitor treatment. MSCs suppressed the proliferation of T cells into tumor tissue. Collectively, these data demonstrate that MSCs directly regulate T cell proliferation by induction of CXCL3 chemokine and its receptor, CXCR2 on the surface in T cells.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Animals, Newborn
Apoptosis drug effects
Apoptosis immunology
Cells, Cultured
Chemokine CXCL1 immunology
Chemokine CXCL1 metabolism
Chemokine CXCL2 immunology
Chemokine CXCL2 metabolism
Chemokines, CXC immunology
Chemokines, CXC metabolism
Chemokines, CXC pharmacology
Cord Blood Stem Cell Transplantation
Flow Cytometry
HeLa Cells
Humans
Janus Kinase 2 immunology
Janus Kinase 2 metabolism
Mesenchymal Stem Cell Transplantation
Mesenchymal Stem Cells metabolism
Mice
Mice, Inbred NOD
Mice, SCID
Microscopy, Fluorescence
Phosphorylation
Receptors, Interleukin-8B metabolism
STAT3 Transcription Factor immunology
STAT3 Transcription Factor metabolism
T-Lymphocytes drug effects
T-Lymphocytes metabolism
Xenograft Model Antitumor Assays methods
Cell Proliferation
Mesenchymal Stem Cells immunology
Receptors, Interleukin-8B immunology
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2163
- Volume :
- 279
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cellular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 23023221
- Full Text :
- https://doi.org/10.1016/j.cellimm.2012.08.002