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A manganese superoxide dismutase with potent antioxidant activity identified from Oplegnathus fasciatus: genomic structure and transcriptional characterization.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2013 Jan; Vol. 34 (1), pp. 23-37. Date of Electronic Publication: 2012 Sep 25. - Publication Year :
- 2013
-
Abstract
- In this study, we describe the identification and characterization of manganese superoxide dismutase, an important antioxidant enzyme acting as the chief reactive oxygen species (ROS) scavenger, from rock bream Oplegnathus fasciatus (Of-mMnSOD) at genomic- and transcriptional-levels as well as the biological activity of recombinant protein. The Of-mMnSOD protein portrayed distinct MnSOD family features including signature motifs, metal association sites and the typical active site topology. It was also predicted to be localized in mitochondrial matrix. The Of-mMnSOD had a quinquepartite genome organization encompassing five exons interrupted by four introns. Comparison of its sequence and gene structure with that of other lineages emphasized its strong conservation among different vertebrates. The Of-mMnSOD was ubiquitously transcribed in different rock bream tissues with higher levels in blood cells and metabolically active tissues. Transcription of Of-mMnSOD was kinetically modulated in response to investigational challenges using mitogens (lipopolysaccharide and poly I:C) and live-pathogens (Edwardsiella tarda and rock bream irido virus) in blood cells and liver tissue. The purified recombinant Of-mMnSOD possessed potential antioxidant capacity and actively survived over a range of pH (7.5-11) and temperature (15-40 °C) conditions. Collectively, findings of this study suggest that Of-mMnSOD combats against oxidative stress and cellular damages induced by mitogen/pathogen-mediated inflammation, by detoxifying harmful ROS (O(2)(●-)) in rock bream.<br /> (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Antioxidants metabolism
Base Sequence
Cloning, Molecular
DNA, Complementary genetics
DNA, Complementary metabolism
Edwardsiella tarda physiology
Fish Proteins chemistry
Fish Proteins immunology
Fish Proteins metabolism
Lipopolysaccharides pharmacology
Molecular Sequence Data
Perciformes immunology
Perciformes metabolism
Phylogeny
Poly I-C pharmacology
Protein Structure, Tertiary
Recombinant Proteins genetics
Recombinant Proteins immunology
Recombinant Proteins metabolism
Sequence Alignment
Superoxide Dismutase chemistry
Superoxide Dismutase immunology
Superoxide Dismutase metabolism
Fish Proteins genetics
Perciformes genetics
Superoxide Dismutase genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 34
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 23022055
- Full Text :
- https://doi.org/10.1016/j.fsi.2012.09.011