[Study on the spatial and temporal expression of beta-AS gene of Glycyrriza uralensis].

Objective: To reveal the temporal and spatial specificity of the expression of beta-AS gene of Glycyrriza uralensis.
Methods: Used PCR to obtain the cDNA of beta-AS gene of Glycyrriza uralensis at different time and from different part and Gel-Pro to carry out the densitometric analysis of the electrophoretic band, then calculate the relative expression.
Results: The spatial specificity experiment showed that beta-AS gene didn't express in the overground part of Glycyrriza uralensis,while in the underground part,the expression of beta-AS in root tip was higher than that of rootstock. And the temporal specificity experiment showed that the expression of beta-AS gene of Glycyrriza uralensis could be divided into 4 stages. From December to February, the expression of beta-AS gene was under the detection limit. From March to May, beta-AS gene began to express. From May to September,the expression of beta-AS gene kept at a high level. And in October and November the expression of beta-AS gene began to decrease.
Conclusion: When the beta-AS gene of Glycyrriza uralensis is researched, the root tip is the suitable plant material and May, June, August and September are the right acquisition time.