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PSAQ™ standards for accurate MS-based quantification of proteins: from the concept to biomedical applications.
- Source :
-
Journal of mass spectrometry : JMS [J Mass Spectrom] 2012 Oct; Vol. 47 (10), pp. 1353-63. - Publication Year :
- 2012
-
Abstract
- Absolute protein quantification, i.e. determining protein concentrations in biological samples, is essential to our understanding of biological and physiopathological phenomena. Protein quantification methods based on the use of antibodies are very effective and widely used. However, over the last ten years, absolute protein quantification by mass spectrometry has attracted considerable interest, particularly for the study of systems biology and as part of biomarker development. This interest is mainly linked to the high multiplexing capacity of MS analysis, and to the availability of stable-isotope-labelled standards for quantification. This article describes the details of how to produce, control the quality and use a specific type of standard: Protein Standard Absolute Quantification (PSAQ™) standards. These standards are whole isotopically labelled proteins, analogues of the proteins to be assayed. PSAQ standards can be added early during sample treatment, thus they can correct for protein losses during sample prefractionation and for incomplete sample digestion. Because of this, quantification of target proteins is very accurate and precise using these standards. To illustrate the advantages of the PSAQ method, and to contribute to the increase in its use, selected applications in the biomedical field are detailed here.<br /> (Copyright © 2012 John Wiley & Sons, Ltd.)
- Subjects :
- Amino Acid Sequence
Buffers
Clinical Chemistry Tests methods
Enterotoxins analysis
Enterotoxins chemistry
Gastrointestinal Contents chemistry
Humans
Hydrogen-Ion Concentration
Insulin-Like Growth Factor I analysis
Insulin-Like Growth Factor I chemistry
Isotopes chemistry
Mass Spectrometry methods
Molecular Sequence Data
Protein Isoforms
Protein Stability
Proteins chemistry
Proteomics methods
Reference Standards
Staphylococcal Infections microbiology
Troponin I analysis
Troponin I chemistry
Clinical Chemistry Tests standards
Mass Spectrometry standards
Proteins analysis
Proteomics standards
Subjects
Details
- Language :
- English
- ISSN :
- 1096-9888
- Volume :
- 47
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of mass spectrometry : JMS
- Publication Type :
- Academic Journal
- Accession number :
- 23019168
- Full Text :
- https://doi.org/10.1002/jms.3106