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Ectopic expression of a maize calreticulin mitigates calcium deficiency-like disorders in sCAX1-expressing tobacco and tomato.
- Source :
-
Plant molecular biology [Plant Mol Biol] 2012 Dec; Vol. 80 (6), pp. 609-19. Date of Electronic Publication: 2012 Sep 25. - Publication Year :
- 2012
-
Abstract
- Deregulated expression of an Arabidopsis H⁺/Ca²⁺ antiporter (sCAX1) in agricultural crops increases total calcium (Ca²⁺) but may result in yield losses due to Ca²⁺ deficiency-like symptoms. Here we demonstrate that co-expression of a maize calreticulin (CRT, a Ca²⁺ binding protein located at endoplasmic reticulum) in sCAX1-expressing tobacco and tomato plants mitigated these adverse effects while maintaining enhanced Ca²⁺ content. Co-expression of CRT and sCAX1 could alleviate the hypersensitivity to ion imbalance in tobacco plants. Furthermore, blossom-end rot (BER) in tomato may be linked to changes in CAX activity and enhanced CRT expression mitigated BER in sCAX1 expressing lines. These findings suggest that co-expressing Ca²⁺ transporters and binding proteins at different intracellular compartments can alter the content and distribution of Ca²⁺ within the plant matrix.
- Subjects :
- Gene Expression
Genes, Plant
Solanum lycopersicum genetics
Solanum lycopersicum metabolism
Plant Diseases genetics
Plants, Genetically Modified
Recombinant Proteins genetics
Recombinant Proteins metabolism
Nicotiana genetics
Nicotiana metabolism
Antiporters genetics
Antiporters metabolism
Calcium metabolism
Calreticulin genetics
Calreticulin metabolism
Cation Transport Proteins genetics
Cation Transport Proteins metabolism
Plant Proteins genetics
Plant Proteins metabolism
Zea mays genetics
Zea mays metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1573-5028
- Volume :
- 80
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Plant molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 23007728
- Full Text :
- https://doi.org/10.1007/s11103-012-9970-6