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The development of a multiplex real-time PCR for the detection of herpes simplex virus 1 and 2, varizella zoster virus, adenovirus and Chlamydia trachomatis from eye swabs.
- Source :
-
Journal of virological methods [J Virol Methods] 2013 Apr; Vol. 189 (1), pp. 143-7. Date of Electronic Publication: 2012 Sep 18. - Publication Year :
- 2013
-
Abstract
- Infectious conjunctivitis can be difficult to distinguish clinically due to the considerable overlap in clinical presentation so clinical diagnosis of conjunctivitis is often insufficient. It is therefore necessary to have a rapid diagnostic test that differentiates between the different causes of infectious conjunctivitis. Screening clinical samples by sample type/syndrome based multiplex real time PCR would allow for rapid detection of a variety of pathogens simultaneously, which will in turn aid in the treatment and clinical management of the patient. A multiplex real-time PCR assay for rapid and simultaneous detection of HSV 1 and 2, VZV, adenovirus and Chlamydia trachomatis (C. trachomatis) from eye swabs was developed and evaluated. The multiplex assay was shown to be sensitive, specific and robust. Reductions in sample turn around times have been achieved by reducing the amount of separate tests needed to be carried out.<br /> (Crown Copyright © 2012. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Adenoviridae genetics
Adenoviridae Infections diagnosis
Chlamydia Infections diagnosis
Chlamydia trachomatis genetics
DNA, Bacterial analysis
DNA, Viral analysis
Female
Herpes Simplex diagnosis
Herpes Zoster diagnosis
Herpesvirus 1, Human genetics
Herpesvirus 1, Human isolation & purification
Herpesvirus 2, Human genetics
Herpesvirus 2, Human isolation & purification
Herpesvirus 3, Human genetics
Herpesvirus 3, Human isolation & purification
Humans
Male
Conjunctivitis, Bacterial diagnosis
Conjunctivitis, Bacterial microbiology
Conjunctivitis, Viral diagnosis
Conjunctivitis, Viral virology
Real-Time Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0984
- Volume :
- 189
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of virological methods
- Publication Type :
- Academic Journal
- Accession number :
- 23000752
- Full Text :
- https://doi.org/10.1016/j.jviromet.2012.08.020