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Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR.
- Source :
-
Journal of veterinary science [J Vet Sci] 2012 Sep; Vol. 13 (3), pp. 323-5. - Publication Year :
- 2012
-
Abstract
- Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.
- Subjects :
- Animals
DNA Primers analysis
Eye virology
Goat Diseases blood
Goat Diseases epidemiology
Goat Diseases virology
Goats
Hair virology
Nose virology
Nucleoproteins analysis
Peste-des-Petits-Ruminants blood
Peste-des-Petits-Ruminants epidemiology
Peste-des-Petits-Ruminants virology
Peste-des-petits-ruminants virus genetics
Pigmentation
RNA, Viral genetics
Reverse Transcriptase Polymerase Chain Reaction standards
Reverse Transcriptase Polymerase Chain Reaction veterinary
Sheep
Sheep Diseases blood
Sheep Diseases epidemiology
Sheep Diseases virology
Uganda epidemiology
Goat Diseases diagnosis
Peste-des-Petits-Ruminants diagnosis
Peste-des-petits-ruminants virus isolation & purification
RNA, Viral isolation & purification
Reverse Transcriptase Polymerase Chain Reaction methods
Sheep Diseases diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1976-555X
- Volume :
- 13
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of veterinary science
- Publication Type :
- Academic Journal
- Accession number :
- 23000590
- Full Text :
- https://doi.org/10.4142/jvs.2012.13.3.323