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Serotype-specific differences in inhibition of reovirus infectivity by human-milk glycans are determined by viral attachment protein σ1.
- Source :
-
Virology [Virology] 2012 Nov 25; Vol. 433 (2), pp. 489-97. Date of Electronic Publication: 2012 Sep 19. - Publication Year :
- 2012
-
Abstract
- Human milk contains many bioactive components, including secretory IgA, oligosaccharides, and milk-associated proteins. We assessed the antiviral effects of several components of milk against mammalian reoviruses. We found that glucocerebroside (GCB) inhibited the infectivity of reovirus strain type 1 Lang (T1L), whereas gangliosides GD3 and GM3 and 3'-sialyllactose (3SL) inhibited the infectivity of reovirus strain type 3 Dearing (T3D). Agglutination of erythrocytes mediated by T1L and T3D was inhibited by GD3, GM3, and bovine lactoferrin. Additionally, α-sialic acid, 3SL, 6'-sialyllactose, sialic acid, human lactoferrin, osteopontin, and α-lactalbumin inhibited hemagglutination mediated by T3D. Using single-gene reassortant viruses, we found that serotype-specific differences segregate with the gene encoding the viral attachment protein. Furthermore, GD3, GM3, and 3SL inhibit T3D infectivity by blocking binding to host cells, whereas GCB inhibits T1L infectivity post-attachment. These results enhance an understanding of reovirus cell attachment and define a mechanism for the antimicrobial activity of human milk.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Capsid Proteins genetics
Cattle
Female
G(M3) Ganglioside immunology
Gangliosides immunology
Genes, Viral
HeLa Cells
Hemagglutination Inhibition Tests
Host-Pathogen Interactions immunology
Humans
L Cells
Mammalian orthoreovirus 3 classification
Mammalian orthoreovirus 3 genetics
Mice
Milk, Human virology
Oligosaccharides immunology
Orthoreovirus, Mammalian classification
Orthoreovirus, Mammalian genetics
Reoviridae Infections immunology
Reoviridae Infections prevention & control
Reoviridae Infections virology
Serotyping
Species Specificity
Virus Attachment
Capsid Proteins immunology
Mammalian orthoreovirus 3 immunology
Mammalian orthoreovirus 3 pathogenicity
Milk, Human immunology
Orthoreovirus, Mammalian immunology
Orthoreovirus, Mammalian pathogenicity
Polysaccharides immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 433
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 22999096
- Full Text :
- https://doi.org/10.1016/j.virol.2012.08.036