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Sildenafil after cardiac arrest and infarction; an experimental rat model.
- Source :
-
Scandinavian cardiovascular journal. Supplement [Scand Cardiovasc J Suppl] 2013 Feb; Vol. 47 (1), pp. 58-64. Date of Electronic Publication: 2012 Oct 10. - Publication Year :
- 2013
-
Abstract
- Objectives: Resuscitation after cardiac arrest may lead to ischemia-reperfusion injury and infarction. We evaluated whether sildenafil, a phosphodiesterase-5 inhibitor, has an impact on recovery after cardiac arrest in a rat cardiac transplantation model.<br />Design: Sixty-one Fischer344 rats underwent syngeneic heterotopic cardiac transplantation after ischemia and ligation of the left anterior coronary artery of the heart to yield myocardial infarction (IRI + MI). Of these, 22 rats received subcutaneously injected sildenafil (1 mg/kg/day) (IRI +MI + S). Twenty-three additional grafted animals with transplantation only served as controls with ischemia reperfusion injury (IRI). After 2 days, immunohistochemistry for eNOS, and RT-PCR for iNOS and Aquaporin-7 were performed after graft harvesting and histology.<br />Results: Two days after transplantation, remote intramyocardial arteries were more preserved in IRI + MI + S as compared with IRI +MI and IRI (0.74 ± 0.14, 0.56 ± 0.23 and 0.55 ± 0.22, PSU, p < 0.05, respectively). Decreased eNOS staining confirmed the presence of developing infarction in IRI + MI and IRI + MI + S. The expression of iNOS was significantly lower during IRI + MI +S as compared with IRI + MI (0.02 ± 0.01 and 1.02 ± 0.02, FC, p < 0.05).<br />Conclusions: Administered at the onset of reperfusion and developing infarction, sildenafil has an impact on myocardial recovery after cardiac arrest and ischemia.
- Subjects :
- Animals
Aquaporins genetics
Coronary Vessels drug effects
Coronary Vessels enzymology
Coronary Vessels pathology
Disease Models, Animal
Drug Administration Schedule
Heart Arrest enzymology
Heart Arrest etiology
Heart Arrest genetics
Heart Arrest pathology
Heart Transplantation
Immunohistochemistry
Injections, Subcutaneous
Myocardial Reperfusion Injury enzymology
Myocardial Reperfusion Injury etiology
Myocardial Reperfusion Injury genetics
Myocardial Reperfusion Injury pathology
Myocardium enzymology
Myocardium pathology
Nitric Oxide Synthase Type II genetics
Nitric Oxide Synthase Type II metabolism
Nitric Oxide Synthase Type III metabolism
Purines administration & dosage
Rats
Rats, Inbred F344
Reverse Transcriptase Polymerase Chain Reaction
Sildenafil Citrate
Time Factors
Heart Arrest drug therapy
Myocardial Infarction complications
Myocardial Reperfusion Injury prevention & control
Phosphodiesterase 5 Inhibitors administration & dosage
Piperazines administration & dosage
Sulfones administration & dosage
Vasodilator Agents administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1651-2510
- Volume :
- 47
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scandinavian cardiovascular journal. Supplement
- Publication Type :
- Academic Journal
- Accession number :
- 22994795
- Full Text :
- https://doi.org/10.3109/14017431.2012.732235