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A real-time PCR detection system for the bois noir and flavescence dorée phytoplasmas and quantification of the target DNA.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 938, pp. 253-68. - Publication Year :
- 2013
-
Abstract
- The real-time PCR detection system for grapevine yellows phytoplasmas described here is composed of two assays for group-specific detection of flavescence dorée (FD) and bois noir (BN) phytoplasmas and a universal phytoplasma assay. It uses hydrolysis minor groove binder probes (TaqMan-MGB). The addition of an assay for amplification of plant DNA co-extracted with phytoplasma DNA provides a further quality control for the DNA extraction and PCR amplification for each sample. The detection system described is reliable, specific, sensitive, and easily applicable to fast, high-throughput diagnosis of grapevine yellows phytoplasmas. In addition to the detection system, an approach for the quantification of phytoplasmas in the sample is described.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 938
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 22987422
- Full Text :
- https://doi.org/10.1007/978-1-62703-089-2_22