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Protective immunity against tick infestation in cattle vaccinated with recombinant trypsin inhibitor of Rhipicephalus microplus.
- Source :
-
Vaccine [Vaccine] 2012 Oct 19; Vol. 30 (47), pp. 6678-85. Date of Electronic Publication: 2012 Sep 07. - Publication Year :
- 2012
-
Abstract
- The cattle tick, Rhipicephalus microplus, is regarded as the most economically important ectoparasite of livestock globally. Control is achieved primarily through the use of acaricides. This approach is hampered by the development of resistance to commercial acaricides among cattle tick populations. Vaccination against R. microplus infestation is another technology that can be integrated for effective cattle tick control. Proteins belonging to the Kunitz-BPTI family are abundant in cattle tick salivary glands, midgut, and ovaries. These organs are attractive targets for the development of a novel cattle tick vaccine. Efficacy assessment against cattle tick infestation in bovines using a vaccine containing the recombinant form of a member of the Kunitz family from R. microplus produced in a yeast expression system is reported for the first time here. The yeast Pichia pastoris was bioengineered to produce the recombinant version of a trypsin inhibitor that is expressed in cattle tick larvae (rRmLTI). Immunization with rRmLTI afforded 32% efficacy against R. microplus. The estimated molecular weight of rRmLTI was 46 kDa. Structural homology to the native form of the larval trypsin inhibitor was documented by recognition of rRmLTI in Western-blots using polyclonal antibodies from mice immunized with cattle tick larval extract or rRmLTI. Bioinformatics analysis of the partial nucleotide and deduced amino acid sequences indicated that the rRmLTI closely resembles BmTI-6, which is a three-headed Kunitz protein present in cattle tick ovary and fat tissue.<br /> (Published by Elsevier Ltd.)
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cattle
Cattle Diseases parasitology
Female
Immunity, Humoral
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Pichia metabolism
Recombinant Proteins immunology
Tick Infestations prevention & control
Vaccination veterinary
Cattle Diseases prevention & control
Rhipicephalus immunology
Tick Infestations veterinary
Trypsin Inhibitors immunology
Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 30
- Issue :
- 47
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 22959980
- Full Text :
- https://doi.org/10.1016/j.vaccine.2012.08.066