Back to Search
Start Over
Cell-penetrating peptide-linked polymers as carriers for mucosal vaccine delivery.
- Source :
-
Molecular pharmaceutics [Mol Pharm] 2012 Oct 01; Vol. 9 (10), pp. 2933-41. Date of Electronic Publication: 2012 Sep 19. - Publication Year :
- 2012
-
Abstract
- We evaluated the potential of poly(N-vinylacetamide-co-acrylic acid) modified with d-octaarginine, which is a typical cell-penetrating peptide, as a carrier for mucosal vaccine delivery. Mice were nasally inoculated four times every seventh day with PBS containing ovalbumin with or without the d-octaarginine-linked polymer. The polymer enhanced the production of ovalbumin-specific immunoglobulin G (IgG) and secreted immunoglobulin A (IgA) in the serum and the nasal cavity, respectively. Ovalbumin internalized into nasal epithelial cells appeared to stimulate IgA production. Ovalbumin transferred to systemic circulation possibly enhanced IgG production. An equivalent dose of the cholera toxin B subunit (CTB), which was used as a positive control, was superior to the polymer in enhancing antibody production; however, dose escalation of the polymer overcame this disadvantage. A similar immunization profile was also observed when ovalbumin was replaced with influenza virus HA vaccines. The polymer induced a vaccine-specific immune response identical to that induced by CTB, irrespective of the antibody type, when its dose was 10 times that of CTB. Our cell-penetrating peptide-linked polymer is a potential candidate for antigen carriers that induce humoral immunity on the mucosal surface and in systemic circulation when nasally coadministered with antigens.
- Subjects :
- Acetamides administration & dosage
Acetamides chemistry
Acetamides immunology
Acrylates administration & dosage
Acrylates chemistry
Acrylates immunology
Adjuvants, Immunologic administration & dosage
Adjuvants, Immunologic chemistry
Administration, Intranasal methods
Animals
Antibody Formation immunology
Antigens administration & dosage
Antigens chemistry
Antigens immunology
Cell-Penetrating Peptides chemistry
Cell-Penetrating Peptides immunology
Cholera Toxin immunology
Drug Carriers administration & dosage
Drug Carriers chemistry
Drug Delivery Systems methods
Epithelial Cells drug effects
Epithelial Cells immunology
Epithelial Cells metabolism
Female
Immunity, Humoral immunology
Immunoglobulin A, Secretory immunology
Immunoglobulin G immunology
Influenza Vaccines administration & dosage
Influenza Vaccines chemistry
Influenza Vaccines immunology
Mice
Mice, Inbred BALB C
Mucous Membrane drug effects
Mucous Membrane immunology
Nasal Mucosa drug effects
Nasal Mucosa immunology
Ovalbumin immunology
Pharmaceutical Solutions administration & dosage
Pharmaceutical Solutions chemistry
Polymers chemistry
Polyvinyls administration & dosage
Polyvinyls chemistry
Vaccination methods
Vaccines chemistry
Cell-Penetrating Peptides administration & dosage
Mucous Membrane metabolism
Nasal Mucosa metabolism
Polymers administration & dosage
Vaccines administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1543-8392
- Volume :
- 9
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Molecular pharmaceutics
- Publication Type :
- Academic Journal
- Accession number :
- 22953762
- Full Text :
- https://doi.org/10.1021/mp300329r