A strategy to enhance the binding affinity of fluorophore-aptamer pairs for RNA tagging with neomycin conjugation.

Authors :: Jeon J
Lee KH
Rao J
Source :: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2012 Oct 14; Vol. 48 (80), pp. 10034-6.
Publication Year :: 2012
Abstract: Fluorogenic sulforhodamine-neomycin conjugates have been designed and synthesized for RNA tagging. Conjugates were fluorescently activated by binding to RNA aptamers and exhibited greater than 250-400 fold enhancement in binding affinity relative to corresponding unconjugated fluorophores.

Subjects :: Binding Sites
Fluorescent Dyes chemical synthesis
Neomycin chemical synthesis
Rhodamines chemical synthesis
Aptamers, Nucleotide chemistry
Fluorescent Dyes chemistry
Neomycin chemistry
Rhodamines chemistry

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