Back to Search
Start Over
A superovulation protocol for the spiny mouse (Acomys cahirinus).
- Source :
-
Reproduction, fertility, and development [Reprod Fertil Dev] 2012; Vol. 24 (8), pp. 1117-22. - Publication Year :
- 2012
-
Abstract
- This study aimed to develop a superovulation protocol for the spiny mouse (Acomys cahirinus). The spiny mouse is a desert-adapted rodent species, with a long oestrus cycle (11 days) compared with rat and mouse, and gives birth to few (mean litter size is 3) precocial offspring after a relatively long gestation (39 days). We successfully optimised a superovulation protocol that elicited a 5-fold increase in the normal ovulation rate of this species. To induce superovulation in the spiny mouse 2 injections of equine chorionic gonadotrophin (eCG, 10 IU each), 9h apart, were required, followed by 20 IU of human chorionic gonadotrophin (hCG). This protocol was successful in 100% of females trialed and at 33 h post-hCG an average of 14.7 ± 1.5, 1-2 cell embryos were recovered. Histological analysis of ovaries following superovulation revealed large corpus lutea and post-ovulatory follicles occupying a large part of the ovary. Ovulation commenced 6-12 h after the hCG injection and continued until 24-33 h post-hCG as indicated by both histological analysis of ovaries and the presence of oocytes/embryos in the oviduct. This superovulation protocol will facilitate the development of an in vitro culture system for spiny mouse embryos.
- Subjects :
- Animals
Chorionic Gonadotropin administration & dosage
Corpus Luteum anatomy & histology
Corpus Luteum drug effects
Female
Gonadotropins, Equine administration & dosage
Litter Size
Ovarian Follicle anatomy & histology
Ovarian Follicle drug effects
Ovary anatomy & histology
Ovary drug effects
Ovulation Induction methods
Murinae physiology
Ovulation Induction veterinary
Superovulation physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1031-3613
- Volume :
- 24
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Reproduction, fertility, and development
- Publication Type :
- Academic Journal
- Accession number :
- 22951246
- Full Text :
- https://doi.org/10.1071/RD12044