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Influence of oxidative stress on inducing micturition dysfunction following chronic infravesical obstruction and the protective role of an antioxidant diet - association of in vivo and in vitro studies in rats.
- Source :
-
International braz j urol : official journal of the Brazilian Society of Urology [Int Braz J Urol] 2012 Jul-Aug; Vol. 38 (4), pp. 552-60. - Publication Year :
- 2012
-
Abstract
- Objective: This study was developed to determine whether the generation of free radicals, induced by ischemia followed by reperfusion in a model of chronic intravesical obstruction in rats, would lead to damage in the detrusor. It also investigates the possible protective action of the flavonoid galangin on the tissue lesion induced by lipid peroxidation.<br />Materials and Methods: Twenty-one male rats were divided into three groups of seven animals each. Group A was subjected to a sham procedure; group B to partial obstruction of the bladder neck; and group C to partial obstruction of the bladder neck, but also received a diet rich in the flavonoid galangin. All the animals were subjected to urodynamic evaluation and then sacrificed. The bladders were sent for enzymatic tests.<br />Results: The urodynamic showed that group B developed significantly greater numbers of involuntary contractions of the detrusor, greater post-micturition residue and lower compliance. The group A presented TEAC levels greater than to the group B. Comparative analysis of group A, B and C demonstrated significantly greater malondialdehyde levels in group B in relation to groups A and C. The group B presented smaller contraction amplitudes than did groups A and C, in electrically stimulated contractions.<br />Conclusions: That oxidative stress is implicated in the damage to the detrusor musculature following a period of chronic intravesical obstruction. We show, for the first time, that administration of an antioxidant prior to and following the start of chronic obstruction makes it possible to avoid the cellular lesions that cause detrusor dysfunction.
- Subjects :
- Animals
Antioxidants pharmacology
Disease Models, Animal
Electrophysiological Phenomena
Flavonoids administration & dosage
Male
Malondialdehyde analysis
Muscle Contraction
Mutagens administration & dosage
Rats
Urinary Bladder enzymology
Urinary Bladder Neck Obstruction physiopathology
Urodynamics
Antioxidants therapeutic use
Oxidative Stress physiology
Urinary Bladder Neck Obstruction etiology
Urination Disorders etiology
Subjects
Details
- Language :
- English
- ISSN :
- 1677-6119
- Volume :
- 38
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- International braz j urol : official journal of the Brazilian Society of Urology
- Publication Type :
- Academic Journal
- Accession number :
- 22951166
- Full Text :
- https://doi.org/10.1590/s1677-55382012000400016