Back to Search
Start Over
Efficient inhibition of porcine reproductive and respiratory syndrome virus replication by artificial microRNAs targeting the untranslated regions.
- Source :
-
Archives of virology [Arch Virol] 2013 Jan; Vol. 158 (1), pp. 55-61. Date of Electronic Publication: 2012 Sep 05. - Publication Year :
- 2013
-
Abstract
- A robust artificial microRNA (amiRNA) strategy against porcine reproductive and respiratory syndrome virus (PRRSV) was developed by targeting the untranslated regions (UTRs). Six candidate amiRNAs targeting the 5' or 3' UTR were used for vector construction, and four effective amiRNAs were selected for further study using a vector transfection/virus infection assay. In cell cultures stably transfected with the four amiRNA vectors, expression of the sequence-specific amiRNAs was confirmed using poly(A)-tailed RT-PCR. After infection with three different PRRSV strains, the viral RNA genome and/or transcript were inhibited by ~90 % (semi-quantitative RT-PCR), and the viral titers were decreased by more than six log CCID(50) (viral titration assay) before day 3 postinfection. The potent anti-PRRSV effects lasted for at least 5 days. Sequence analysis showed that the amiRNA antiviral activities were not compromised by the presence of one or two mismatches in their binding targets. This work constitutes a step towards developing a more effective RNAi strategy against PRRSV.
- Subjects :
- Animals
Base Sequence
Cell Line
Down-Regulation
MicroRNAs metabolism
Molecular Sequence Data
Porcine respiratory and reproductive syndrome virus physiology
RNA Interference
RNA, Viral metabolism
Swine
MicroRNAs genetics
Porcine Reproductive and Respiratory Syndrome virology
Porcine respiratory and reproductive syndrome virus genetics
RNA, Viral genetics
Untranslated Regions
Virus Replication
Subjects
Details
- Language :
- English
- ISSN :
- 1432-8798
- Volume :
- 158
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 22948796
- Full Text :
- https://doi.org/10.1007/s00705-012-1455-z