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Family-based linkage and association mapping reveals novel genes affecting Plum pox virus infection in Arabidopsis thaliana.
- Source :
-
The New phytologist [New Phytol] 2012 Nov; Vol. 196 (3), pp. 873-886. Date of Electronic Publication: 2012 Sep 03. - Publication Year :
- 2012
-
Abstract
- Sharka is a devastating viral disease caused by the Plum pox virus (PPV) in stone fruit trees and few sources of resistance are known in its natural hosts. Since any knowledge gained from Arabidopsis on plant virus susceptibility factors is likely to be transferable to crop species, Arabidopsis's natural variation was searched for host factors essential for PPV infection. To locate regions of the genome associated with susceptibility to PPV, linkage analysis was performed on six biparental populations as well as on multiparental lines. To refine quantitative trait locus (QTL) mapping, a genome-wide association analysis was carried out using 147 Arabidopsis accessions. Evidence was found for linkage on chromosomes 1, 3 and 5 with restriction of PPV long-distance movement. The most relevant signals occurred within a region at the bottom of chromosome 3, which comprises seven RTM3-like TRAF domain-containing genes. Since the resistance mechanism analyzed here is recessive and the rtm3 knockout mutant is susceptible to PPV infection, it suggests that other gene(s) present in the small identified region encompassing RTM3 are necessary for PPV long-distance movement. In consequence, we report here the occurrence of host factor(s) that are indispensable for virus long-distance movement.<br /> (© 2012 INRA. New Phytologist © 2012 New Phytologist Trust.)
- Subjects :
- Agrobacterium tumefaciens genetics
Agrobacterium tumefaciens metabolism
Alleles
Arabidopsis immunology
Arabidopsis metabolism
Arabidopsis virology
Arabidopsis Proteins genetics
Arabidopsis Proteins metabolism
Chromosomes, Plant genetics
Chromosomes, Plant metabolism
Crosses, Genetic
Disease Resistance
Gene Knockout Techniques
Genes, Plant
Genetic Association Studies methods
Microsatellite Repeats
Plant Diseases genetics
Plant Diseases immunology
Plant Diseases virology
Plum Pox Virus immunology
Plum Pox Virus metabolism
Pollination
Polymorphism, Single Nucleotide
Quantitative Trait Loci
Arabidopsis genetics
Chromosome Mapping methods
Genetic Linkage
Genome, Plant
Plum Pox Virus pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8137
- Volume :
- 196
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The New phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 22943366
- Full Text :
- https://doi.org/10.1111/j.1469-8137.2012.04289.x