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Increased shear stress inhibits angiogenesis in veins and not arteries during vascular development.
- Source :
-
Angiogenesis [Angiogenesis] 2013 Jan; Vol. 16 (1), pp. 71-83. Date of Electronic Publication: 2012 Sep 02. - Publication Year :
- 2013
-
Abstract
- Vascular development is believed to occur first by vasculogenesis followed by angiogenesis. Though angiogenesis is the formation of new vessels, we found that vascular density actually decreases during this second stage. The onset of the decrease coincided with the entry of erythroblasts into circulation. We therefore measured the level of shear stress at various developmental stages and found that it was inversely proportional to vascular density. To investigate whether shear stress was inhibitory to angiogenesis, we altered shear stress levels either by preventing erythroblasts from entering circulation ("low" shear stress) or by injection of a starch solution to increase the blood plasma viscosity ("high" shear stress). By time-lapse microscopy, we show that reverse intussusception (merging of two vessels) is inversely proportional to the level of shear stress. We also found that angiogenesis (both sprouting and splitting) was inversely proportional to shear stress levels. These effects were specific to the arterial or venous plexus however, such that the effect on reverse intussusception was present only in the arterial plexus and the effect on sprouting only in the venous plexus. We cultured embryos under altered shear stress in the presence of either DAPT, a Notch inhibitor, or DMH1, an inhibitor of the bone morphogenetic protein (BMP) pathway. DAPT treatment phenocopied the inhibition of erythroblast circulation ("low" shear stress) and the effect of DAPT treatment could be partially rescued by injection of starch. Inhibition of the BMP signaling prevented the reduction in vascular density that was observed when starch was injected to increase shear stress levels.
- Subjects :
- Animals
Blood Viscosity
Bone Morphogenetic Proteins metabolism
Coturnix embryology
Coturnix physiology
Embryo, Mammalian blood supply
Embryo, Mammalian physiology
Embryo, Nonmammalian blood supply
Embryo, Nonmammalian physiology
Intussusception embryology
Intussusception physiopathology
Mice
Receptors, Notch metabolism
Regional Blood Flow
Signal Transduction
Time-Lapse Imaging
Arteries embryology
Arteries physiology
Neovascularization, Physiologic
Stress, Mechanical
Veins embryology
Veins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-7209
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Angiogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 22941228
- Full Text :
- https://doi.org/10.1007/s10456-012-9300-2