Back to Search
Start Over
In vivo electroporation improves therapeutic potency of a DNA vaccine targeting hepadnaviral proteins.
In vivo electroporation improves therapeutic potency of a DNA vaccine targeting hepadnaviral proteins.
- Source :
-
Virology [Virology] 2012 Nov 10; Vol. 433 (1), pp. 192-202. Date of Electronic Publication: 2012 Aug 24. - Publication Year :
- 2012
-
Abstract
- This preclinical study investigated the therapeutic efficacy of electroporation (EP)-based delivery of plasmid DNA (pDNA) encoding viral proteins (envelope, core) and IFN-γ in the duck model of chronic hepatitis B virus (DHBV) infection. Importantly, only DNA EP-therapy resulted in a significant decrease in mean viremia titers and in intrahepatic covalently closed circular DNA (cccDNA) levels in chronic DHBV-carrier animals, compared with standard needle pDNA injection (SI). In addition, DNA EP-therapy stimulated in all virus-carriers a humoral response to DHBV preS protein, recognizing a broader range of major antigenic regions, including neutralizing epitopes, compared with SI. DNA EP-therapy led also to significant higher intrahepatic IFN-γ RNA levels in DHBV-carriers compared to other groups, in the absence of adverse effects. We provide the first evidence on DNA EP-therapy benefit in terms of hepadnaviral infection clearance and break of immune tolerance in virus-carriers, supporting its clinical application for chronic hepatitis B.<br /> (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Antibodies, Viral biosynthesis
Antibodies, Viral immunology
Chronic Disease
DNA, Circular genetics
DNA, Circular immunology
Disease Models, Animal
Ducks
Electroporation
Epitopes
Hepadnaviridae Infections immunology
Hepadnaviridae Infections prevention & control
Hepadnaviridae Infections virology
Hepatitis B Vaccines immunology
Hepatitis, Viral, Animal immunology
Hepatitis, Viral, Animal virology
Immune Tolerance
Immunity, Humoral
Interferon-gamma biosynthesis
Interferon-gamma immunology
Plasmids
Vaccines, DNA immunology
Viral Core Proteins genetics
Viral Envelope Proteins genetics
Viremia immunology
Viremia prevention & control
Viremia veterinary
Viremia virology
Hepadnaviridae Infections veterinary
Hepatitis B Vaccines administration & dosage
Hepatitis B Virus, Duck immunology
Hepatitis, Viral, Animal prevention & control
Vaccines, DNA administration & dosage
Viral Core Proteins immunology
Viral Envelope Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 433
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 22921316
- Full Text :
- https://doi.org/10.1016/j.virol.2012.07.014