Back to Search
Start Over
Fc engineering: design, expression, and functional characterization of antibody variants with improved effector function.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2012; Vol. 907, pp. 519-36. - Publication Year :
- 2012
-
Abstract
- Today monoclonal antibodies are widely used in cancer therapy. However, clinical experience as well as translational research into antibodies' pharmacology and effector mechanisms has identified limitations of antibody therapy, including inefficient effector cell recruitment or initiation of complement-dependent cytotoxicity (CDC). These insights opened alleys for further improvement of antibodies' immunomodulatory functions. While second generation antibodies were predominantly engineered to reduce immunogenicity, progress in antibody engineering now enables the generation of antibodies with novel interesting features. The introduction of Fc engineering technologies offers the potential to tailor Fc-mediated effector functions such as antibody-dependent cell-mediated cytotoxicity (ADCC), CDC or phagocytosis. Approaches to improve Fc-mediated effector mechanisms by Fc-engineering allow for the design of so-called "fit-for-purpose" antibodies or antibody-derivatives, hopefully overcoming some limitations of current forms of antibody therapy.
- Subjects :
- Animals
Antibody-Dependent Cell Cytotoxicity immunology
Base Sequence
Blotting, Western
CHO Cells
Cell Death
Cricetinae
Cricetulus
Electrophoresis, Polyacrylamide Gel
Genetic Vectors genetics
Glycolysis
Humans
Immunoglobulin G biosynthesis
Lectins metabolism
Models, Molecular
Molecular Sequence Data
Mutagenesis, Site-Directed
Protein Binding
Receptors, Fc metabolism
Recombinant Fusion Proteins biosynthesis
Recombinant Fusion Proteins isolation & purification
Single-Chain Antibodies biosynthesis
Staining and Labeling
Antibodies metabolism
Immunoglobulin Fc Fragments biosynthesis
Protein Engineering methods
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 907
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 22907372
- Full Text :
- https://doi.org/10.1007/978-1-61779-974-7_30