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Effect of Shengmai injection () on diaphragmatic contractility in doxorubicin-treated rats.
- Source :
-
Chinese journal of integrative medicine [Chin J Integr Med] 2014 Jan; Vol. 20 (1), pp. 43-8. Date of Electronic Publication: 2012 Aug 18. - Publication Year :
- 2014
-
Abstract
- Objective: To explore the diaphragmatic toxicity in doxorubicin (DOX)-treated rats and the related mechanisms, as well as the effects of Shengmai Injection (SMI, ) on the diaphragmatic dysfunction.<br />Methods: Thirty Sprague-Dawley male rats were randomly divided into three groups: control, DOX-treated and DOX+SMI treated groups. DOX was given to rats in DOX and DOX+SMI groups in 6 equal doses [2.5 mg/kg, intraperitoneal injection (i.p.)], on alternate days, over a period of 2 weeks for a cumulative dose of 15 mg/kg. SMI was given to DOX+SMI rats in 12 doses (3 mL/kg, i.p.) for a period of 2 weeks before the administration of DOX and 2 weeks during the administration of DOX. The rats in the control group received equal volume of normal saline. Subsequently, the twitch and tetanic characteristics and force-frequency relationships, and the malondialdehyde (MDA) levels and the superoxide dismutase (SOD) activities, as well as the mRNA content and proteins of inducible nitric oxide synthase (iNOS) were determined.<br />Results: The DOX-treated rats had decreased the peak twitch tension (Pt), maximal tetanic tension (P0) and force-frequency relationship as compared with the control rats (P<0.01), while the diaphragm contractility in rats treated with SMI were significantly higher than that in DOX-treated rats (P<0.01). The DOX-treated rats had increased MAD levels and decreased SOD activities (P<0.05), and SMI decreased the MDA levels and increased the SOD activities in DOX-treated rats (P<0.05). Ultrastructure of diaphragm in the DOX-treated rats revealed typical alterations including fracture of diaphragm fibers, and edema and degeneration of mitochondria; these changes were relieved by SMI treatment. The mRNA content and protein of iNOS in DOX-treated rats were remarkably higher than those in control rats (P<0.01), while SMI decreased the mRNA expression level of iNOS in DOX-treated rats (P<0.05).<br />Conclusions: Lipid peroxidation is responsible for DOX-induced diaphragm toxicity. SMI protects diaphragm muscles and their function from DOX impairment, and these beneficial effects may be somehow correlated with the decrease in expression of iNOS and lipid peroxidation.
- Subjects :
- Animals
Biomechanical Phenomena drug effects
Blotting, Western
Diaphragm pathology
Diaphragm ultrastructure
Drug Combinations
Gene Expression Regulation drug effects
In Vitro Techniques
Injections
Male
Malondialdehyde metabolism
Nitric Oxide Synthase Type II genetics
Nitric Oxide Synthase Type II metabolism
Oxidative Stress drug effects
RNA, Messenger genetics
RNA, Messenger metabolism
Rats
Rats, Sprague-Dawley
Diaphragm drug effects
Diaphragm physiology
Doxorubicin adverse effects
Drugs, Chinese Herbal pharmacology
Muscle Contraction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1993-0402
- Volume :
- 20
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Chinese journal of integrative medicine
- Publication Type :
- Academic Journal
- Accession number :
- 22903440
- Full Text :
- https://doi.org/10.1007/s11655-012-1096-9