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Trophoblastic cell lines ACH1P and AC-1M32 react with a distinctive cytokine pattern toward Listeria monocytogenes and show morphologic differences.
- Source :
-
American journal of reproductive immunology (New York, N.Y. : 1989) [Am J Reprod Immunol] 2012 Nov; Vol. 68 (5), pp. 387-91. Date of Electronic Publication: 2012 Aug 14. - Publication Year :
- 2012
-
Abstract
- Problem: The differential reaction of cytotrophoblast and syncytiotrophoblast towards infection with listeria monocytogenes (LM) is pivotal to its pathogenicity. In this study we tested the cytokine signature upon infection with listeria monocytogenes (LM) in an in vitro model.<br />Method of Study: We compared two related trophoblastic cell lines (AC-1M32 and ACH1P). The cell line ACH1P showed syncytium formation, whereas AC-1M32 did not fuse, as demonstrated with immunfluorescence E-Cadherin staining. In a Multi-Analyte ELISArray we tested for concentrations of TNFα, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12, IL-13, IL-17A, IL-8, MCP1, MIP-1a, MIP-1b, MDC, Eotaxin, IFNγ, G-CSF, TGFβ1 after 8 and 24 hours.<br />Results: Compared to unstimulated cells, the syncytial cell line ACH1P showed a significant induction of Interleukin-6 (IL-6), Monocyte Chemotactic Protein-1 (MCP-1) and transforming growth factor β-1 (TGFβ1). Incubating AC-1M32 with LM, however, showed significantly reduced IL-6 levels and a massively increased (~300fold) TGFβ1 secretion compared to unstimulated controls.<br />Conclusions: A functional anti-LM immune response was only induced by the syncytium-forming cell line ACH1P. Using the two sister cell lines AC-1M32 and ACH1P in an in vitro LM-stimulation assay might facilitate research in the area of placental listeria infection.<br /> (© 2012 John Wiley & Sons A/S.)
- Subjects :
- Cell Line
Chemokine CCL2 metabolism
Enzyme-Linked Immunosorbent Assay
Female
Fluorescent Antibody Technique
Humans
Interleukin-6 metabolism
Listeria monocytogenes immunology
Listeriosis microbiology
Transforming Growth Factor beta metabolism
Trophoblasts ultrastructure
Cytokines biosynthesis
Giant Cells ultrastructure
Listeria monocytogenes pathogenicity
Listeriosis immunology
Trophoblasts immunology
Trophoblasts microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0897
- Volume :
- 68
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- American journal of reproductive immunology (New York, N.Y. : 1989)
- Publication Type :
- Academic Journal
- Accession number :
- 22892046
- Full Text :
- https://doi.org/10.1111/aji.12004