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Understanding the antibacterial mechanism of CuO nanoparticles: revealing the route of induced oxidative stress.
- Source :
-
Small (Weinheim an der Bergstrasse, Germany) [Small] 2012 Nov 05; Vol. 8 (21), pp. 3326-37. Date of Electronic Publication: 2012 Aug 13. - Publication Year :
- 2012
-
Abstract
- To date, there is still a lack of definite knowledge regarding the interaction of CuO nanoparticles with bacteria and the possible permeation of the nanoparticles into bacterial cells. This study was aimed at shedding light on the size-dependent (from the microscale down to the small nanoscale) antibacterial activity of CuO. The potent antibacterial activity of CuO nanoparticles was found to be due to ROS-generation by the nanoparticles attached to the bacterial cells, which in turn provoked an enhancement of the intracellular oxidative stress. This paradigm was confirmed by several assays such as lipid peroxidation and reporter strains of oxidative stress. Furthermore, electron microscopy indicated that the small nanoparticles of CuO penetrated the cells. Collectively, the results reported herein may reconcile conflicting concepts in the literature concerning the antibacterial mechanism of CuO nanoparticles, as well as highlight the potential for developing sustainable CuO nanoparticles-based devices for inhibiting bacterial infections.<br /> (Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Adenosine Triphosphate metabolism
Animals
Colony Count, Microbial
Copper chemistry
Electron Spin Resonance Spectroscopy
Escherichia coli drug effects
Escherichia coli growth & development
Escherichia coli ultrastructure
HEK293 Cells
HeLa Cells
Humans
Intracellular Space drug effects
Intracellular Space metabolism
Lipid Peroxidation drug effects
Mice
Microbial Sensitivity Tests
Microbial Viability drug effects
Particle Size
Staphylococcus aureus drug effects
Staphylococcus aureus growth & development
Staphylococcus aureus ultrastructure
Superoxides metabolism
Anti-Bacterial Agents pharmacology
Copper pharmacology
Metal Nanoparticles chemistry
Oxidative Stress drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1613-6829
- Volume :
- 8
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Small (Weinheim an der Bergstrasse, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 22888058
- Full Text :
- https://doi.org/10.1002/smll.201200772