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Optogenetic field potential recording in cortical slices.
- Source :
-
Journal of neuroscience methods [J Neurosci Methods] 2012 Sep 30; Vol. 210 (2), pp. 119-24. Date of Electronic Publication: 2012 Jul 31. - Publication Year :
- 2012
-
Abstract
- We introduce a method that uses optogenetic stimulation to evoke field potentials in brain slices prepared from transgenic mice expressing channelrhodopsin-2-YFP. Cortical slices in a recording chamber were stimulated with a 473 nm blue laser via either a laser scanning photostimulation setup or by direct guidance of a fiber optic. Field potentials evoked by either of the two optogenetic stimulation methods had stable amplitude, consistent waveform, and similar components as events evoked with a conventional stimulating electrode. The amplitude of evoked excitatory postsynaptic potentials increased with increasing laser intensity or pulse duration. We further demonstrated that optogenetic stimulation can be used for the induction and monitoring of long-term depression. We conclude that this technique allows for efficient and reliable activation of field potentials in brain slice preparation, and will be useful for studying short and long term synaptic plasticity.<br /> (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Bacterial Proteins genetics
Cerebral Cortex metabolism
Channelrhodopsins
Dose-Response Relationship, Radiation
Evoked Potentials genetics
Evoked Potentials radiation effects
In Vitro Techniques
Lasers
Long-Term Potentiation genetics
Long-Term Potentiation physiology
Long-Term Potentiation radiation effects
Long-Term Synaptic Depression genetics
Long-Term Synaptic Depression radiation effects
Luminescent Proteins genetics
Mice
Mice, Transgenic
Photic Stimulation methods
Cerebral Cortex physiology
Evoked Potentials physiology
Optogenetics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1872-678X
- Volume :
- 210
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of neuroscience methods
- Publication Type :
- Academic Journal
- Accession number :
- 22884773
- Full Text :
- https://doi.org/10.1016/j.jneumeth.2012.07.019