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FHL2 inhibits the Id3-promoted proliferation and invasive growth of human MCF-7 breast cancer cells.
- Source :
-
Chinese medical journal [Chin Med J (Engl)] 2012 Jul; Vol. 125 (13), pp. 2329-33. - Publication Year :
- 2012
-
Abstract
- Background: Id3 plays a key role in the progression of breast cancer. Previously, four and a half LIM protein (FHL2) was identified as a repressor of Id family proteins by interacting with them. This study aimed to investigate the effects of FHL2 on the transcriptional regulation and oncogenic activities of Id3 in human breast cancer cells.<br />Methods: Cell transfection was performed with SuperFect reagent. Stable transfectants that overexpressed Id3 were obtained by selection on G418. The level of Id3 protein was determined by Western blotting analysis. Dual luciferase assays were used to measure the effect of Id3 and FHL2 on E47-mediated transcriptional activity in MCF-7 human breast cancer cells. The MTT assay was used to measure cell proliferation. The transwell assay was used to measure the invasive capacity of MCF-7 cancer cells.<br />Results: Id3 markedly repressed transcription mediated by the basic helix-loop-helix (bHLH) factor E47 in MCF-7 cells. This Id3-mediated repression was effectively antagonized by FHL2. Overexpression of Id3 markedly promoted the proliferation and invasive capacity of MCF-7 cells; however, these effects were significantly suppressed by the overexpression of FHL2.<br />Conclusions: FHL2 can inhibit the proliferation and invasive growth of human breast cancer cells by repressing the functional activity of Id3. The functional roles of FHL2-Id3 signaling in the development of human breast cancer need further research.
- Subjects :
- Blotting, Western
Breast Neoplasms genetics
Cell Line, Tumor
Cell Proliferation
Humans
Inhibitor of Differentiation Proteins genetics
LIM-Homeodomain Proteins genetics
MCF-7 Cells
Muscle Proteins genetics
Neoplasm Proteins genetics
Transcription Factor 3 genetics
Transcription Factor 3 metabolism
Transcription Factors genetics
Breast Neoplasms metabolism
Inhibitor of Differentiation Proteins metabolism
LIM-Homeodomain Proteins metabolism
Muscle Proteins metabolism
Neoplasm Proteins metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2542-5641
- Volume :
- 125
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Chinese medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 22882857