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Exon 8 amplification of epidermal growth factor receptor (EGFR) in invasive breast carcinomas.
- Source :
-
Histopathology [Histopathology] 2012 Oct; Vol. 61 (4), pp. 644-51. - Publication Year :
- 2012
-
Abstract
- Aims: The rate of EGFR amplification in breast cancer ranges between 0% and 15%. Recent studies have focused on the amplification status of cytosine-adenine (CA) repeats in intron 1 of the gene and correlated it with increased EGFR protein. The aim of this study was to investigate, for the first time, the significance of coding exon 8 amplification of EGFR in invasive breast cancer.<br />Methods and Results: We investigated, by means of real-time polymerase chain reaction (PCR), the amplification status of exon 8 of the EGFR gene in 148 paraffin-embedded tissue sections, 115 with invasive breast carcinoma and 33 controls. Immunohistochemistry was utilized to detect EGFR and human epidermal growth factor receptor 2 (HER2) expression. Univariate and multivariate statistical analyses were performed for the evaluation of our results. EGFR amplification was observed in 7.8% of the patients, and EGFR was immunodetected in 9.6%. EGFR amplification was correlated positively with EGFR expression (Pā<ā0.0001), HER2 expression (P = 0.023), coexpression of EGFR/HER2 (Pā<ā0.0001) and nuclear grade (P = 0.047), and inversely with ER protein expression (P = .047).<br />Conclusion: It appears that amplification of the coding sequence exon 8 exhibits similar biological behaviour to amplification of the regulatory sequence in intron 1, leading to elevated levels of EGFR protein.<br /> (© 2012 Blackwell Publishing Ltd.)
Details
- Language :
- English
- ISSN :
- 1365-2559
- Volume :
- 61
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Histopathology
- Publication Type :
- Academic Journal
- Accession number :
- 22882128
- Full Text :
- https://doi.org/10.1111/j.1365-2559.2012.04315.x