Back to Search
Start Over
In vitro ligament-bone interface regeneration using a trilineage coculture system on a hybrid silk scaffold.
- Source :
-
Biomacromolecules [Biomacromolecules] 2012 Sep 10; Vol. 13 (9), pp. 2692-703. Date of Electronic Publication: 2012 Aug 10. - Publication Year :
- 2012
-
Abstract
- The ligament-bone interface is a complex structure that comprises ligament, fibrocartilage, and bone. We hypothesize that mesenchymal stem cells cocultured in between ligament and bone cells, on a hybrid silk scaffold with sections suitable for each cell type, would differentiate into fibrocartilage. The section of scaffold for osteoblast seeding was coated with hydroxyapatite. A trilineage coculture system (osteoblasts-BMSCs-fibroblasts) on a hybrid silk scaffold was established. RT-PCR results and immunohistochemistry results demonstrated that BMSCs cocultured between fibroblasts and osteoblasts had differentiated into the fibrocartilaginous lineage. The morphological change was also observed by SEM observation. A gradual transition from the uncalcified to the calcified region was formed in the cocultured BMSCs from the region that directly interacted with fibroblasts to the region that directly interacted with osteoblasts. The role of transforming growth factor β3 (TGF-β3) in this trilineage coculture model was also investigated by supplementing the coculture system with 10 ng/mL TGF-β3. The TGF-treated group showed similar results of fibrocartilaginous differentiation of BMSCs with coculture group without TGF-β3 supplement. However, no calcium deposition was found in the cocultured BMSCs in the TGF-treated group. This may indicate TGF-β3 delayed the mineralization process of chondrocytes.
- Subjects :
- Animals
Biomarkers metabolism
Bone Marrow Cells drug effects
Bone Marrow Cells metabolism
Bone Regeneration drug effects
Bone and Bones cytology
Cell Differentiation drug effects
Coated Materials, Biocompatible pharmacology
Coculture Techniques
Durapatite
Fibroblasts drug effects
Fibroblasts metabolism
Fibrocartilage cytology
Fibrocartilage drug effects
Fibrocartilage growth & development
Ligaments cytology
Ligaments drug effects
Ligaments growth & development
Mesenchymal Stem Cells drug effects
Mesenchymal Stem Cells metabolism
Microscopy, Electron, Scanning
Osteoblasts drug effects
Osteoblasts metabolism
Primary Cell Culture
Rabbits
Tissue Engineering
Tissue Scaffolds
Transforming Growth Factor beta3 pharmacology
Bone Marrow Cells cytology
Coated Materials, Biocompatible chemistry
Fibroblasts cytology
Mesenchymal Stem Cells cytology
Osteoblasts cytology
Silk chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1526-4602
- Volume :
- 13
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Biomacromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 22880933
- Full Text :
- https://doi.org/10.1021/bm300651q