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Preparation and characterization of a fully active biotinylated probe of cholecystokinin.
- Source :
-
Preparative biochemistry [Prep Biochem] 1990; Vol. 20 (3-4), pp. 205-17. - Publication Year :
- 1990
-
Abstract
- In this study we describe the synthesis and purification of biotinylated cholecystokinin-8 (Bio-CCK-8) and characterize its use as a probe for the pancreatic cholecystokinin receptor. CCK-8 (0.1 umoles) was reacted with either radiolabeled d-[8,9(-3)H]biotin succinimide ester (0.5 umoles) or N-hydroxysuccinimidyl-biotin in dimethylformamide and triethylamine, and purified by anion exchange chromatography. Concentrations of Bio-CCK-8 and CCK-8 needed for half-maximal inhibition of [125]I-CCK-8 binding to pancreatic membranes were the same (1.0 and 1.3 nM). Bio-CCK-8 retained full biological activity as determined by stimulation of pancreatic protein secretion from rats, and the biotin group bound to CCK-8 retained its high sensitivity for avidin.
Details
- Language :
- English
- ISSN :
- 0032-7484
- Volume :
- 20
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Preparative biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 2287607
- Full Text :
- https://doi.org/10.1080/00327489008050197