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Targeted multiplex imaging mass spectrometry with single chain fragment variable (scfv) recombinant antibodies.
- Source :
-
Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2012 Oct; Vol. 23 (10), pp. 1689-96. Date of Electronic Publication: 2012 Aug 07. - Publication Year :
- 2012
-
Abstract
- Recombinant scfv antibodies specific for CYP1A1 and CYP1B1 P450 enzymes were combined with targeted imaging mass spectrometry to simultaneously detect the P450 enzymes present in archived, paraffin-embedded, human breast cancer tissue sections. By using CYP1A1 and CYP1B1 specific scfv, each coupled to a unique reporter molecule (i.e., a mass tag) it was possible to simultaneously detect multiple antigens within a single tissue sample with high sensitivity and specificity using mass spectrometry. The capability of imaging multiple antigens at the same time is a significant advance that overcomes technical barriers encountered when using present day approaches to develop assays that can simultaneously detect more than a single antigen in the same tissue sample.
- Subjects :
- Animals
Antibodies, Monoclonal chemistry
Antibodies, Monoclonal immunology
Aryl Hydrocarbon Hydroxylases chemistry
Aryl Hydrocarbon Hydroxylases immunology
Avidin chemistry
Avidin immunology
Biotin chemistry
Biotin immunology
Breast chemistry
Breast Neoplasms chemistry
Cytochrome P-450 CYP1A1 chemistry
Cytochrome P-450 CYP1A1 immunology
Cytochrome P-450 CYP1B1
Enzyme-Linked Immunosorbent Assay
Female
Histocytochemistry methods
Humans
Mice
Rats
Recombinant Proteins chemistry
Recombinant Proteins metabolism
Sensitivity and Specificity
Single-Chain Antibodies metabolism
Mass Spectrometry methods
Molecular Imaging methods
Single-Chain Antibodies chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1123
- Volume :
- 23
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of the American Society for Mass Spectrometry
- Publication Type :
- Academic Journal
- Accession number :
- 22869296
- Full Text :
- https://doi.org/10.1007/s13361-012-0423-x