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Acute mechanical forces cause deterioration in lung structure and function in elastase-induced emphysema.
- Source :
-
American journal of physiology. Lung cellular and molecular physiology [Am J Physiol Lung Cell Mol Physiol] 2012 Oct 01; Vol. 303 (7), pp. L567-74. Date of Electronic Publication: 2012 Aug 03. - Publication Year :
- 2012
-
Abstract
- The relation between the progression of chronic obstructive pulmonary disease (COPD) and exacerbations is unclear. Currently, no animal model of acute exacerbation of COPD (AECOPD) exists. The objectives of this study were to evaluate the effects of mechanical forces induced by deep inspirations (DIs) on short-term deterioration of lung structure and function to mimic AECOPD. At 2, 7, or 21 days after treatment with elastase, mice were ventilated with or without DIs (35 cmH(2)O airway pressure for 3 s, 2 times/min) for 1 h. Functional residual capacity (FRC) was measured with body plethysmography, and respiratory compliance, resistance, and hysteresivity were obtained via forced oscillations. From hematoxylin and eosin-stained sections, equivalent airspace diameters (D), alveolar wall thickness (W(t)), number of septal ruptures (N(sr)), and attachment density (A(d)) around airways were determined. FRC, compliance, and hysteresivity statistically significantly increased with time, and both increased due to DIs. Interestingly, DIs also had an effect on FRC, compliance, resistance, and hysteresivity in control mice. The development of emphysema statistically significantly increased D and W(t) in time, and the DIs caused subtle differences in D. At 21 days, the application of DIs changed the distribution of D, increased W(t) and N(sr), and decreased A(d). These results suggest that once a critical remodeling of the parenchyma has been reached, acute mechanical forces lead to irreversible changes in structure and function, mimicking COPD exacerbations. Thus, the acute application of DIs in mice with emphysema may serve as a useful model of AECOPD.
- Subjects :
- Airway Resistance drug effects
Animals
Disease Models, Animal
Disease Progression
Lung Compliance drug effects
Male
Mice
Mice, Inbred C57BL
Pancreatic Elastase pharmacology
Pulmonary Disease, Chronic Obstructive chemically induced
Pulmonary Emphysema chemically induced
Respiratory Function Tests
Pulmonary Disease, Chronic Obstructive pathology
Pulmonary Disease, Chronic Obstructive physiopathology
Pulmonary Emphysema pathology
Pulmonary Emphysema physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1504
- Volume :
- 303
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Lung cellular and molecular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 22865550
- Full Text :
- https://doi.org/10.1152/ajplung.00217.2012