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[Effect of flavin adenine dinucleotide on ultraviolet B induced damage in cultured human corneal epithelial cells].
- Source :
-
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan [Yakugaku Zasshi] 2012; Vol. 132 (8), pp. 933-7. - Publication Year :
- 2012
-
Abstract
- This study evaluated the effects of flavin adenine dinucleotide (FAD) on ultraviolet B (UV-B)-induced damage in cultured human corneal epithelial (HCE-T) cells. The cultured HCE-T cells were treated with 0.003125-0.05% FAD before exposure to 80 mJ/cm2 UV-B. Cell viability was measured 24 h after UV-B irradiation using the MTS assay. Reactive oxygen species (ROS) were detected 30 min after UV-B irradiation using 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate acetyl ester. Apoptosis was evaluated 4 h after UV-B irradiation in the caspase-3/7 activity assay. UV-B irradiation reduced cell viability and stimulated ROS production and caspase-3/7 activity in HCE-T cells. Pretreatment of UV-B irradiated HCE-T cells with FAD significantly attenuated cell viability reduction and inhibited the stimulation of both ROS production and caspase-3/7 activity due to UV-B exposure compared with those with vehicle (0% FAD). These results clarified that FAD inhibits ROS-mediated apoptosis by UV-B irradiation in HCE-T cells and suggest that FAD may be effective as a radical scavenger in UV-B-induced corneal damage.
- Subjects :
- Caspase 3 metabolism
Caspase 7 metabolism
Cell Survival drug effects
Cell Survival radiation effects
Cells, Cultured
Cornea enzymology
Cornea radiation effects
Epithelial Cells enzymology
Epithelial Cells radiation effects
Humans
Ultraviolet Rays
Cornea drug effects
Epithelial Cells drug effects
Flavin-Adenine Dinucleotide pharmacology
Subjects
Details
- Language :
- Japanese
- ISSN :
- 1347-5231
- Volume :
- 132
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
- Publication Type :
- Academic Journal
- Accession number :
- 22864352
- Full Text :
- https://doi.org/10.1248/yakushi.132.933