Back to Search
Start Over
Self-illuminating quantum dots for highly sensitive in vivo real-time luminescent mapping of sentinel lymph nodes.
- Source :
-
International journal of nanomedicine [Int J Nanomedicine] 2012; Vol. 7, pp. 3433-43. Date of Electronic Publication: 2012 Jul 05. - Publication Year :
- 2012
-
Abstract
- Quantum dots (QDs) show promise as novel nanomaterials for sentinel lymph node (SLN) mapping through their use in noninvasive in vivo fluorescence imaging, and they have provided remarkable results. However, in vivo fluorescence imaging has limitations mainly reflected in the strong autofluorescence and low deepness of tissue penetration associated with this technique. Here, we report on the use of self-illuminating 3-mercaptopropionic acid-capped CdTe/CdS QDs for mouse axillary SLN mapping by bioluminescence resonance energy transfer, which was found to overcome these limitations [corrected]. We used CdTe/CdS QDs synthesized in aqueous solution to conjugate a mutant of the bioluminescent protein, Renilla reniformis luciferase. The nanobioconjugates obtained had an average hydrodynamic diameter of 19 nm, and their luminescence catalyzed by the substrate (coelenterazine) could penetrate into at least 20 mm of hairless pigskin, which could be observed using an in vivo imaging system equipped with a 700 nm emission filter. Conversely, the fluorescence of the nanobioconjugates penetrated no more than 10 mm of pigskin and was observed with a strong background. When 80 μL of the nanobioconjugates (containing about 0.5 μmol/L of QDs) and 5 μL of coelenterazine (1 μg/μL) were intradermally injected into a mouse paw, the axillary SLN could be imaged in real time without external excitation, and little background interference was detected. Furthermore, the decayed luminescence of QD-Luc8 in SLNs could be recovered after being intradermally reinjected with the coelenterazine. Our data showed that using self-illuminating QDs, as opposed to fluorescence QDs, has greatly enhanced sensitivity in SLN mapping, and that the SLN could be identified synchronously by the luminescence and fluorescence of the self-illuminating QDs.
- Subjects :
- 3-Mercaptopropionic Acid chemistry
Animals
Cadmium Compounds chemistry
Fluorescent Dyes administration & dosage
Fluorescent Dyes pharmacokinetics
Injections, Intradermal
Luminescent Measurements methods
Lymph Nodes metabolism
Mice
Nanoconjugates administration & dosage
Optical Imaging methods
Skin chemistry
Skin Absorption
Sulfides chemistry
Swine
Tellurium chemistry
Whole Body Imaging
Fluorescent Dyes chemistry
Lymph Nodes chemistry
Nanoconjugates chemistry
Quantum Dots
Sentinel Lymph Node Biopsy methods
Spectrometry, Fluorescence methods
Subjects
Details
- Language :
- English
- ISSN :
- 1178-2013
- Volume :
- 7
- Database :
- MEDLINE
- Journal :
- International journal of nanomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 22848169
- Full Text :
- https://doi.org/10.2147/IJN.S30709