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Role of metallothionein 1E in the migration and invasion of human glioma cell lines.
- Source :
-
International journal of oncology [Int J Oncol] 2012 Oct; Vol. 41 (4), pp. 1305-13. Date of Electronic Publication: 2012 Jul 24. - Publication Year :
- 2012
-
Abstract
- Metallothionein 1E (MT1E) has been found to be highly expressed in motile cell lines. We investigated whether MT1E actually modulates the migration and invasion of human glioma cell lines and the types of factors that have an effect on MT1E. RNA differential display was performed using Genefishing™ technology in the human glioma cell lines U343MG-A, U87MG and U87MG-10'; the results were validated by RT-PCR and northern blot analysis, in order to detect possible genetic changes as the determining factors for migration ability in malignant glioma. MT1E was identified in U87MG, a highly motile cell line. The migration and invasion abilities of human glioma cell lines, and MT1E transfectants were investigated using simple scratch testing and Matrigel invasion assays. Morphological and cytoskeletal (actin, vimentin) changes were documented by light and confocal microscopy. The expression of MT1E in four glioma cell lines was assessed by RT-PCR and western blotting. In addition, the effects of MT1E on the activity of the NF-κB p50/p65 transcription factor, MMP-2 and -9 were examined by western blotting and zymography. The endogenous MT1E expression in the human glioma cell lines was statistically correlated with their migratory abilities and invasion. The U87-MT-AS cells became more round and had decreased stress fibers, compared with the U87MG cells. Endogenous MT1E expression in the four human glioma cell lines was directly correlated with migration. Two antisense MT1E-transfected cell lines showed decreased NF-κB p50 translocation into the nucleus, which led to decreased activity of MMP-9 in conditioned media. It may be postulated that MT1E can enhance the migration and invasion of human glioma cells by inducing MMP-9 inactivation via the upregulation of NF-κB p50.
- Subjects :
- Brain Neoplasms metabolism
Brain Neoplasms pathology
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Glioma pathology
Humans
Matrix Metalloproteinase 2 metabolism
Matrix Metalloproteinase 9 metabolism
Metallothionein metabolism
NF-kappa B p50 Subunit metabolism
Transcriptional Activation
Transfection
Brain Neoplasms genetics
Cell Movement genetics
Glioma genetics
Metallothionein genetics
Neoplasm Invasiveness genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2423
- Volume :
- 41
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- International journal of oncology
- Publication Type :
- Academic Journal
- Accession number :
- 22843066
- Full Text :
- https://doi.org/10.3892/ijo.2012.1570