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Recombinatorial biases and convergent recombination determine interindividual TCRβ sharing in murine thymocytes.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2012 Sep 01; Vol. 189 (5), pp. 2404-13. Date of Electronic Publication: 2012 Jul 23. - Publication Year :
- 2012
-
Abstract
- Overlap of TCR repertoires among individuals provides the molecular basis for public T cell responses. By deep-sequencing the TCRβ repertoires of CD4+CD8+ thymocytes from three individual mice, we observed that a substantial degree of TCRβ overlap, comprising ∼10-15% of all unique amino acid sequences and ∼5-10% of all unique nucleotide sequences across any two individuals, is already present at this early stage of T cell development. The majority of TCRβ sharing between individual thymocyte repertoires could be attributed to the process of convergent recombination, with additional contributions likely arising from recombinatorial biases; the role of selection during intrathymic development was negligible. These results indicate that the process of TCR gene recombination is the major determinant of clonotype sharing between individuals.
- Subjects :
- Animals
CD4-Positive T-Lymphocytes cytology
CD4-Positive T-Lymphocytes immunology
CD4-Positive T-Lymphocytes metabolism
CD8-Positive T-Lymphocytes cytology
CD8-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes metabolism
Female
Mice
Random Allocation
Receptors, Antigen, T-Cell, alpha-beta genetics
Thymus Gland cytology
Cell Differentiation immunology
Receptors, Antigen, T-Cell, alpha-beta physiology
Recombination, Genetic immunology
Thymus Gland immunology
Thymus Gland metabolism
V(D)J Recombination immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 189
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 22826324
- Full Text :
- https://doi.org/10.4049/jimmunol.1102087