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Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag.
- Source :
-
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2012 Sep 07; Vol. 48 (69), pp. 8694-6. Date of Electronic Publication: 2012 Jul 24. - Publication Year :
- 2012
-
Abstract
- The micro-viscosity and molecular crowding experienced by specific proteins can regulate their dynamics and function within live cells. Taking advantage of the emerging TMP-tag technology, we present the design, synthesis and application of a hybrid genetic-chemical molecular rotor probe whose fluorescence lifetime can report protein-specific micro-environments in live cells.
- Subjects :
- Carbocyanines analysis
Carbocyanines metabolism
Cell Survival
Escherichia coli genetics
Fluorescent Dyes analysis
HEK293 Cells
Histones analysis
Histones genetics
Histones metabolism
Humans
Recombinant Fusion Proteins analysis
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Tetrahydrofolate Dehydrogenase analysis
Tetrahydrofolate Dehydrogenase metabolism
Transfection
Trimethoprim analysis
Viscosity
Escherichia coli enzymology
Fluorescent Dyes metabolism
Microscopy, Fluorescence methods
Tetrahydrofolate Dehydrogenase genetics
Trimethoprim metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1364-548X
- Volume :
- 48
- Issue :
- 69
- Database :
- MEDLINE
- Journal :
- Chemical communications (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 22825488
- Full Text :
- https://doi.org/10.1039/c2cc33133k