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Estrogen modulates hepatic gene expression and survival of rainbow trout infected with pathogenic bacteria Yersinia ruckeri.
- Source :
-
Marine biotechnology (New York, N.Y.) [Mar Biotechnol (NY)] 2012 Oct; Vol. 14 (5), pp. 530-43. Date of Electronic Publication: 2012 Jul 24. - Publication Year :
- 2012
-
Abstract
- In the aquatic environment, fish are exposed to various stimuli at once and have developed different response mechanisms to deal with these multiple stimuli. The current study assessed the combined impacts of estrogens and bacterial infection on the physiological status of fish. Juvenile rainbow trout were exposed to two different concentrations of 17β-estradiol (E2) (2 or 20 mg/kg feed) and then infected with three concentrations of Yersinia ruckeri, a bacterial pathogen causing massive losses in wild and farmed salmonid populations. Organism-level endpoints to assess the impact of the single and combined treatments included hepatic vitellogenin transcript expression to evaluate the E2 exposure efficiency and survival rate of pathogen-challenged fish. The two E2 doses increased vitellogenin levels within the physiological range. Infection with Y. ruckeri caused mortality of trout, and this effect was significantly enhanced by a simultaneous exposure to high E2 dose. The hormone reduced survival at intermediate and high (10(4) and 10(6) colony forming units, cfu) bacterial concentrations, but not for a low one (10(2) cfu). Analysis of hepatic gene expression profiles by a salmonid 2 k cDNA microarray chip revealed complex regulations of pathways involved in immune responses, stress responses, and detoxicification pathways. E2 markedly reduced the expression of several genes implicated in xenobiotic metabolism. The results suggest that the interaction between pathogen and E2 interfered with the fish's capability of clearing toxic compounds. The findings of the current study add to our understanding of multiple exposure responses in fish.
- Subjects :
- Animals
Colony Count, Microbial veterinary
DNA Primers genetics
Dose-Response Relationship, Drug
Microarray Analysis veterinary
Real-Time Polymerase Chain Reaction veterinary
Reverse Transcriptase Polymerase Chain Reaction
Survival Analysis
Vitellogenins metabolism
Yersinia Infections drug therapy
Estradiol pharmacology
Fish Diseases drug therapy
Fish Diseases microbiology
Gene Expression Regulation drug effects
Oncorhynchus mykiss
Yersinia Infections veterinary
Yersinia ruckeri
Subjects
Details
- Language :
- English
- ISSN :
- 1436-2236
- Volume :
- 14
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Marine biotechnology (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 22825393
- Full Text :
- https://doi.org/10.1007/s10126-012-9473-0