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Overcoming drug resistance with alginate oligosaccharides able to potentiate the action of selected antibiotics.
- Source :
-
Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2012 Oct; Vol. 56 (10), pp. 5134-41. Date of Electronic Publication: 2012 Jul 23. - Publication Year :
- 2012
-
Abstract
- The uncontrolled, often inappropriate use of antibiotics has resulted in the increasing prevalence of antibiotic-resistant pathogens, with major cost implications for both United States and European health care systems. We describe the utilization of a low-molecular-weight oligosaccharide nanomedicine (OligoG), based on the biopolymer alginate, which is able to perturb multidrug-resistant (MDR) bacteria by modulating biofilm formation and persistence and reducing resistance to antibiotic treatment, as evident using conventional and robotic MIC screening and microscopic analyses of biofilm structure. OligoG increased (up to 512-fold) the efficacy of conventional antibiotics against important MDR pathogens, including Pseudomonas, Acinetobacter, and Burkholderia spp., appearing to be effective with several classes of antibiotic (i.e., macrolides, β-lactams, and tetracyclines). Using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), increasing concentrations (2%, 6%, and 10%) of alginate oligomer were shown to have a direct effect on the quality of the biofilms produced and on the health of the cells within that biofilm. Biofilm growth was visibly weakened in the presence of 10% OligoG, as seen by decreased biomass and increased intercellular spaces, with the bacterial cells themselves becoming distorted and uneven due to apparently damaged cell membranes. This report demonstrates the feasibility of reducing the tolerance of wound biofilms to antibiotics with the use of specific alginate preparations.
- Subjects :
- Acinetobacter drug effects
Acinetobacter genetics
Burkholderia drug effects
Burkholderia genetics
Drug Interactions
Drug Resistance, Bacterial genetics
Enterobacteriaceae drug effects
Enterobacteriaceae genetics
Genotype
Glucuronic Acid chemistry
Hexuronic Acids chemistry
Microbial Sensitivity Tests
Oligosaccharides chemistry
Pseudomonas aeruginosa drug effects
Pseudomonas aeruginosa genetics
Staphylococcus aureus drug effects
Staphylococcus aureus genetics
Streptococcus oralis drug effects
Streptococcus oralis genetics
Alginates chemistry
Anti-Bacterial Agents pharmacology
Oligosaccharides pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-6596
- Volume :
- 56
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Antimicrobial agents and chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 22825116
- Full Text :
- https://doi.org/10.1128/AAC.00525-12