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Bryophyllum pinnatum inhibits detrusor contractility in porcine bladder strips--a pharmacological study towards a new treatment option of overactive bladder.
- Source :
-
Phytomedicine : international journal of phytotherapy and phytopharmacology [Phytomedicine] 2012 Jul 15; Vol. 19 (10), pp. 947-51. Date of Electronic Publication: 2012 Jul 21. - Publication Year :
- 2012
-
Abstract
- Aims: A broad spectrum of synthetic agents is available for the treatment of overactive bladder. Anti-cholinergic drugs show a poor compliance due to side effects. There is an increasing use of plant extracts in medicine. We have therefore investigated the inhibitory effects of leaf press juice from Bryophyllum pinnatum (Lam.) Oken (Kalanchoe pinnata L.) on bladder strips and compared the effects to that of oxybutynin.<br />Methods: Strips of porcine detrusor were prepared in Krebs solution and contractility was measured in a myograph system chamber aired with O₂/CO₂ at 37 °C. To induce contractions, electrical field stimulation (32 Hz, 40 V) was used for the inhibitory effect measurements, and carbachol (50 μM) for the relaxant effect measurements. Recordings were obtained in the absence and presence of increasing concentrations of Bryophyllum pinnatum leaf press juice (BPJ, 0.1-10%), and oxybutynin (10⁻⁷-10⁻³ M) as a reference substance.<br />Results: In inhibition experiments, BPJ as well as oxybutynin inhibited electrically induced contractions of porcine detrusor. BPJ at concentrations of 5% inhibited the contraction compared to a time matched control significantly by 74.6±10.2% (p<0.001). BPJ as well as oxybutynin relaxed carbachol pre-contracted porcine detrusor strips. The maximum relaxant effect of BPJ compared to a time matched control was 18.7±3.7 (p<0.05) at a concentration of 10% BPJ.<br />Conclusions: Our investigations show that BPJ inhibits contractions induced by electrical field stimulation and relaxes carbachol-induced contractions. However, the effect was lower than that of the reference substance oxybutynin. It is important to continue in vitro experiments as well as clinical studies with BPJ that might offer a new treatment option for patients with OAB.<br /> (Copyright © 2012 Elsevier GmbH. All rights reserved.)
- Subjects :
- Animals
Carbachol pharmacology
Cholinergic Agonists pharmacology
Electric Stimulation
Mandelic Acids pharmacology
Muscarinic Antagonists pharmacology
Muscarinic Antagonists therapeutic use
Muscle, Smooth physiology
Parasympatholytics pharmacology
Phytotherapy
Plant Leaves
Plant Preparations pharmacology
Plant Preparations therapeutic use
Swine
Urinary Bladder physiology
Kalanchoe
Mandelic Acids therapeutic use
Muscle Contraction drug effects
Muscle, Smooth drug effects
Parasympatholytics therapeutic use
Urinary Bladder drug effects
Urinary Bladder, Overactive drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1618-095X
- Volume :
- 19
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 22824522
- Full Text :
- https://doi.org/10.1016/j.phymed.2012.05.003